资源描述：
《左卡尼汀对丙戊酸钠诱导的大鼠急性肝损伤的保护作用研》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。
1、...佳木斯大学硕士学位论文ProtectiveeffectsofL-carnitinetovalproate-inducedacuteliverinjuryinratsAbstractObjective:ToinvestigatetheprotectiveeffectofL-carnitinevalproate-inducedacuteliverinjuryinyoungrats.Methods:VPAinducedinyoungratswithacuteliverinjuryinducedbydifferentdosesofL-carniti
2、neandbiochemicalindexescontrastchange,alterliverantioxidantindexandpathologybeforeandaftertreatment.Establishmentofacuteliverinjurymodelofratsinducedbysodiumvalproate，theratswererandomlydividedinto5groups，normalgroup,modelgroup,andthreegroupsofLC(0.25,0.5g,1.0g/kg).Thenormalg
3、roupwasgivenequalvolumeofdistilledwater,themodelgroupgavageofsodiumvalproate100mg/kg,LCgroupsweregavageofsodiumvalproatein100mg/kg,onegroupreceivedLC0.25g/kg,secondgroupweregivenLC0.5g/kg,thethirdgroupwasgivenLC1.0g/kg,administered1timesdaily,for21days.At24hafterthelastadmini
4、stration,rattailveinblood2ml.,usingALTandASTkitandOLYMPUSmultifunctionautomaticbiochemicalanalyzerforthedeterminationofbiochemicalindexinserumalanineaminotransferase(ALT)andaspartateaminotransferase(AST)level.Detectionoflivertissue,usingthemethodofxanthineoxidaseandsuperoxide
5、dismutase(Superoxide,Dismutase,SOD)activityandmalondialdehyde(MDA)content.Theanatomyofratlivertissue,andpathologicalobservationoflivertissueofeyeviewofchangepartofthelivertissuewastakenandfixedin10%formalin,embeddedinparaffin,slicedandstainedwithHE,observedthepathologicalchan
6、gesofliverunderlightmicroscope.Results:Successfullyestablishedtheliverpathologicaldamagemodelinducedbysodiumvalproate.Modelgroupcomparedwiththenormalgroup,theserumlevelsofALT,ASTwereincreasedsignificantly.AfteroraladministrationofLCinacuteliverinjuryanimalserumALT,ASTweredecr
7、eased,inadose-dependentmanner(P1＞0.05，P2、3＜0.05),thelevelofSODinlivertissuesofratswereincreaseddosedependently(P1＞0.05，P2、3＜0.05),andthecontentof-5-......万方数据......佳木斯大学硕士学位论文MDAincreasedwiththedoseofLCdecreased(P1＞0.05，P2、3＜0.05),reducethepathologicalchangesoflivertissueofra
8、tseffectively.Conclusion:TheprotectiveeffectofLConliverinjuryinduced
