红曲菌氨肽酶的分析

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1、ABSTRACTMonascuscanproduceavarietyofbiologicallyactiveenzymes：amylase,glucoamylase,maltase,protease,pectinase,lipase,esterenzyme,carboxypeptidase,aminopeptidaseenzymesandSOon．Aminopeptidaseisaprotease，whichplayaimportantroleindebitteringproteinhydrolyzateandthedep

2、thhydrolysisofproteins．Relativetoextractaminopeptidaseformanimalsandplants，microbialfermentationofaminopeptidasehastheadvantagesoflow-cost，largeoutput，highactivity．ThispaperfocusesonsolidstatefermentationandliquidsstatefermentationofaminopeptidasebyMonascus，screen

3、ingouttheoptimalconditionsforproductionofaminopeptidase．aminopeptidasewerepurifiedbyingusingtwoaqueousphaseextractionandultrafiltrationandweexploratedtheprocesswhichcouldenlargetheextractionofaminopeptidase．Inaddition，wepreliminarystudiedtheenzymaticpropertiesofpu

4、rifiedaminopeptidase．Solid。statefermentationandliquidfermenmtionconditionofaminopeptidasebyMonascuswereoptimized，enzymeproductionconditionsoptimization，theresultsshowedthat：liquidfermentation．ofaminopeptidasecostedrelativelyshortertime．Andenzymeactivityreachedthem

5、aximumactivityafter7days，whichwas376．9U／mL．ontheotherhand，enzymebySolidstatefermentationafter13dayswhichwas115．3U／mL：HowtopurifycrudeaminopeptidasesolutioninMonascusfermentationproductswasstudiedandtheresultsshowedthataftermakingLinearamplificationexperimentOfopti

6、mizedtwoaqueousphaseextractionsystembasedoncrudesolutionproducingbysolidformentation，proteinpartitioncoefficientKp，enzymepurificationcoefficientKandtherecoveryrateofenzymewerealmostdoesnotchange，indicatingthatthissystemcanbeexpandusedforaminopeptidasecrudeenzymeex

7、traction．Inaccordancewiththeconditionsoftheaqueoustwo—phasesystem：pH7．5，themassfractionof18％(NH4)2S04，themassfractionof18％PEG一2000，zoominaqueoustwo’phasesystem，500times，respectivelyextractingthecrudeenzymeAandliquidfermentationproductsfromthecrudeenzymeBinthe1000m

8、LsolidIIIfermentationproductextractionbyultrafiltrationwiththeoptimumpressure0．1Mpa，30kDapolyethersulfonehollowfibermembraneswereconcentratedtwotimestot