人羊膜匀浆上清液治疗兔角膜碱烧伤的实验的研究

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1、第三军医大学硕士学位论文英文缩写NBSFBSD／F12rpmHAMbFGFFNTGF—BPBSMTTOD英文缩写一览表英文全称NovelbovineserumFoetalbovineserunlDulbeccoModifiedEagle’SF12HAMBaseMediumnutrientmixtureRevolutionperminuteHtlmanAmniotieMembranebasicfibroblastgrowthfactorfibrotintransforminggrowthfactorbetaphosphatebufferedsa

2、linemethylthiotetrazoleopticaldensit中文全称新生小牛血清胎牛血清DMEM／F12培养基每分钟转速人羊膜碱性成纤维细胞生长因子纤维连接蛋白转化生长因子6磷酸缓冲液四甲基偶氮唑盐光密度值第三军医大学硕士学使论文ExperimentalStudyonHumanAmnioticHomogenatetoCornealHealingafterAlkaliBurnofCornealinRatsAbstractobjectiveToselecteffectivemethodsforeyealkalinebumbyevalu

3、atingtheeffectsofsupernatantaofhomogenizedfreshamrfiontorabbitcomealepithelialcellandalkalinebumofrabbicorIlea．Methods1．Experimentinvitro：Therabbitcornealepithelialcellwerecultured．Weobservedtheeffectsofsupernatantaofhomogenizedfreshamnionatdifferentconcentrationf40、80、1609

4、9／m1)andatdifferenthours(24、48．96h)onrabbitcornealepithelialcellbytetrazotium(MTT)colorimetricassay．2。Experimentinvivo：fifteenrabbits(thirtyeyes)wererandomlydividedintocontrolgroups(teneyes)、experimentgroups(supernatantaofhomogenizedfreshamnion，teneyes)andbFGFgroups(teneyes

5、)．Themodelofalkalineburnofrabbitcorneaweresetup．Cornealangiogenesisandinepitheliumwereobservedwithaslitlampmicroscope．Theratioofneovascularizationareaandepitheliumdefecttocornea’swerecalculated．ThespecimenwastakentostainwithHE。VGandAgNORsindifferenttimeaftercautery，Thenumbe

6、rofthefibroblastwascalculatedwithmicroscope．TheexpressionofFNincorneawasdetectedwithimmunohistochemicalassay。Results：1．Proliferationrateofhomogenateamniononrabbitcornealepithelialcelt：Rabbitcornealepithelialcellcallproliferateatdifferenthours(24、49、96hour)andatdifferentconc

7、entrationofsupernatantaofhomogenizedamnion(40rtg／m1．80btg／ml、1601』g／m1)．2．Thehealingofepithelium：ThehealingareaofepitheliumofexperimentgroupsandbFGFgroupswasmorethancontrolgroups(Patfirstday．seconddayandthirdday．Thedifferencewassignificantatfouthdaytoseventhday(P<0，01

8、)．3。霹拈cornealneovascularization：髓嚣differenceofthearea蕊comealneovascularizationallo