骨化三醇对实验性大鼠骨性关节炎关节软骨和软骨下骨的影响

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1、宁夏医科大学硕士研究生学位论文中文摘要组（P<0.01），两组软骨细胞MMP-13表达强度均较ACLT+V组低（P<0.05），ACLT+L组和ACLT+H组软骨下骨骨量均较ACLT+V组减少（P<0.01）。ACLT+L组和ACLT+H组间各指标比较差异无统计学意义（P>0.05）。结论1.大鼠前交叉韧带切断能够造成动物膝关节软骨OA样改变，表现出软骨下骨骨吸收、骨重建的变化特点。2.骨代谢调节剂骨化三醇通过抑制OA早期软骨下骨骨吸收，可减缓OA后期发生的软骨下骨增生硬化，从而对关节软骨发挥一定保护作用。3.软骨下骨在OA关节软骨退变早期即可发生形

2、态学改变，干预OA病变早期软骨下骨异常改变可减缓OA病理进展。关键词骨关节炎，基质金属蛋白酶-13，骨化三醇，动物模型，软骨下骨，骨组织形态计量学II宁夏医科大学硕士研究生学位论文英文摘要TheeffectofcalcitriolonarticularcartilageandsubcondralboneinratsexperimentalosteoarthritisABSTRACTObjectiveThepurposeofthisstudyis：1.toestablishaosteoarthritismodelinratkneesbytransact

3、inganteriorcruciateligament,andcharacterizingsubchondralboneremodeling,cartelagedamageduringthediseaseprogressioninthemodelsofsurgicallyinducedOA；2.toobservetheeffectofcalcitioloncartilageandsubchondralboneintheratanteriorcruciateligamenttransactionmodel,andassesstheroleofsubch

4、ondralboneintheetiologyofosteoarthritis(OA).MethodExperimentone:Thirtyratsweredividedintothreegroup(2weekspostsurgery、6weekspost-surgeryand10weekspost-surgerygroup).Rats’rightkneejointsweresubjectedtoanteriorcruciateligamenttransection(ACLT),whiletheleftkneeserveascontrol.Thera

5、tsweresacrificedandthetibilplateswereharvestedwhichwerestudiedinbothgross-morphologicalandpathohistological,atthesametimeCoimmunostainingformatrixmetaloproteinase13(MMP-13)wasperformedtoinvestigatetheprogressionofcartilagedegradetion.Experimentwo:sixty-fourratsweredividedintofo

6、uegroups:sham，anteriorcruciateligamenttransaction(ACLT),ACLT+Lowdosecalcitiol(ACLT+L)andACLT+Highdosecalcitiol(ACLT+H)group.Afteroperation,ratsin-1-1groupACLT+LandgroupACLT+Hreceivedcalcitriol0.025μg/kg/dayand0.1-1-1μg/kg/dayrespectivelyintragastricadministrationallyeverydayfor

7、thefollowing2weeks.Theratsweresacrificedat2weeksand10weekspostsurgery,andthetibilplateswereharvestedwhichwerestudiedinbothpathohistologicalandbonehistomorphometricanalysis.AtthesametimeCoimmunostainingformatrixmetalloproteinase13(MMP-13)wasperformedtoinvestigatetheprogressionof

8、cartilagedegradetion.ResultExperimentone:Thedegenerati