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ID:32376897
大小:5.65 MB
页数:42页
时间:2019-02-03
《不同底物诱导下桦剥管菌纤维素酶活和蛋白质组学研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、万方数据AbstractCellulaseandhemicellulasecanbesecretedbywoodbrownrotfungi,whichwasplayanimportantr01einthecycleofnaturecellulose.Piptoporusbetulinusisakindoftypicalbrownrotfungi.TherewaslittleresearchaboutthedegradationmechanismofPiptoporusbetulinus·Inordertoinvestigatetheligocellu
2、loseactivitiesproducedbyPiptoporusbetulinusandthedifferencesofenzyme.relatedproteinexpression,measuredofdifferentwood(birch,spruce)inducedculturetubestrippingbirchfungusgrowth,cellulaseactivityandintracellularproteinexpressionwerecompared,andpreliminaryexplorationonexpressionof
3、extracellularproteinsPtptoporusbetulinuswereculturedon23。Cand28‘C,measuredthediameterofcolonyevery24hours.Theaveragegrowthrateat23*Cwas8.9mln/dand28。Cwas13turn/d·TheresuItsshowedthatPiptoporusbetulinuswasmoresuitableforincubatedat28℃.Birchchips.spruceschipsandwheatbranwereinduc
4、er,potatoliquidmediumwithoutwoodchipsasthecontr01,determinedtheactivityofcellulasewithin12days.Theactivityofendo-cellulasewasthehighest,exo—cellulasefollowed,p-glucosidasewasthelowest·Theactivitvofendo—cellulaseinthefirst9daysshowedarisingtrend,whiletheactivityofexo。cellulasewa
5、sveryhighontheearlytraining,withthetimeincreasetheactivitybegantodecline.atier9daystheactivitybegantoincreased.Theactivityofp。glucosidasewasverylOWin9days,itwasobviouslydetectedonthe12days.ThefungalintracellularproteomechangesofPiptoporusbetulinusinducedbythesawdustofdifferentt
6、reespeciesisdetectedby2-DEandtandemtwo—dimensionalelectrophoresis-masssDectrometryanalysis.Comparedwiththecontrol(withoutwoodchips),32proteinswithgreatdifferentialexpressioninthetreatmentgroup.17proteinspotswassuccessfullyidentifiedthroughtheMALDITOF/TOF,13proteinsup-regulated,
7、4proteinspotsdownregulated·TheseDroteinshadavarietyofmolecularfunctions,suchastransferases,hydrolases,proteinbinding,signaltransduction,etc.,andrelatestothebiosynthesis,catabolism,thestimuli十responsive,nitrogen..containingcompoundsandothermetabolicprocesses·Extractedtheextracel
8、lularsecretedproteinsonthe15thday,Proteinprofilesweree
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