检测肺癌患者外周血和骨髓微转移的临床意义

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1、维普资讯http://www.cqvip.com·62中华肿瘤杂志2生!筮箜鲞箜塑』．临床研究．检测肺癌患者外周血和骨髓微转移的临床意义周清华宫友陵覃扬孙芝琳孙泽芳刘伦旭李潞朱文【摘要】目的探讨检测肺癌患者外周血和骨髓微转移的特异性、敏感性及临床意义。方法应用巢式RT-PCR技术，检测59例肺癌患者、11例肺部良性病变患者和20例健康人外周血、骨髓中CK19mRNA表达。结果巢式RT-PCR检测技术的敏感性达到10-6。59例肺癌患者中，加例检测出外周血微转移，l3例检测出骨髓微转移，其阳性检出率分别为33．9％(20／59)和22

2、．0％(13／59)，二者有显著正相关(P<0．05)。肺癌患者外周血和骨髓微转移与肺癌组织学类型、细胞分化程度及P-TNM分期均存在密切关系(P<0．05或P<0．01)。肺良性病变患者、健康人外周血和骨髓中均未检测出CK19mRNA表达。结论肺癌患者外周血和骨髓中均存在用常规方法检测不出的微转移；应用巢式RT-PCR技术检测肺癌患者外周血和骨髓中CK19mRNA表达，对肺癌微转移的分子诊断具有重要的理论意义和临床应用前景。【主题词】肺肿瘤；癌，非小细胞肺；肿瘤转移；聚合酶链反应Valueofa~noangmicrometasta

3、sisbynestedR11-PCRintheperipheralbloodandbonemaI1innon-smallcelllungcancerpatientszHOUQtf曾hun，GONGYou-ling，QINYang，sUNZhi-lin，sUNZe-fang，，JH，13，ZHUn、Laboratoryo厂Z枷CancerMo~cularBiology，CancerCenterandCancerlmtitutetChinaHospit，SwhuanUniversity，Chengdu610041，China【Abstr

4、act】ObjectiveToevaluatethespecificity，sensitivityandtheirclinicalsignificanceofdetectingCK19mRNAexpressionbvnestedRrr_pCRformoleculardiagnosisofmicmmetastasisintheperipheralbloodandbonerflalTowofpatientswithnon-smallcellungcancer．MethodsCK19ⅡlRNAexpressionWaSdetectedby

5、nestedRT—PCRinperipheralbloodandboneIIIalTOWsamplesfrom59lungcancerpatients，withsamplesofllt-e印pulmonarylesionpatientsand20heal山yadultsascontro1．ResultsThesensitivityofnestedr-PCRWaSl0_。。．rhepositiveratesofmicrometastasiswere33．89％(20／59)inperipheralbloodand22．03％(13／5

6、9)inbonemarrow，withahighlypositivecorrelationexistingbetweenthetwogroups(P<0．05)．Ikmicrometastasisiflperipheralbloodandbonemal'l~WwaScloselycorrelatedwiththepathologiealelassifcationandcelldiferentiation(P<0．05)andp-TNMstage(P<0．01)．NoCK19mRNAexpressionWaSfoundinthesam

7、plesfrompatientswb-epulmonarylesionorhealthyadultvolunteers．ConclusionnIeperipheralbloodandbonemanowfrompatientswithnon—smallcellungcancerpossessesmierometastasisthatCannotbedetectedbycommonmethocls．NestedRT-PCRtechniqueshowsfavorablespecificityandsensitivityindetectin

8、gtheconditionwithdefiniteclinicalprospects．【SubjectWOldS】IamgneoplaSms；Carcinoma，non-smallcalllung；Neoplasmmetastasis