bfgf联合骨髓间充质干细胞促进周围神经再生的实验研究

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1、THEEXPERIM匣NTALSTUDYOFbF(讯JOINTM匣SENCHYMAI，STEMCELLSFORPROMOTINGPERIPHERALNERVEI也(逦NERATIONResearchorientation：bonesurgeryPostgraduatestudent：LiDaweiGraduatestudenttutor：LiBingshengABSTRACTObjective：Toobservethebasicfibroblastgrowthfactorjointmesenchymalstemcell

2、sinducedperipheralnerveregenerationrolefortheclinicalencountercasesofperipheralnervedefectstof'mdawayofconvenientandfeasiblerestoration．Methods：1、MSCscollection,trainingWeuseketaminetoanesthetizeaWistarrats，andcutapartlateralSkillofthighinsterileconditions，strip

3、pingoutthefemur,andwashmarrowcavityrepeatly、7I，imheparinphysiologicalsaline，thenfluidintothetube，mixandcentrifuge，washtwicebyD-Hanksbalancedsaltsolution，andthencentrifugeagain，jointhebasisofvaccinationintheculturemediumbottleat37℃，volumeWasO．05ofC02saturationhum

4、idityfortraining．Replacebalancedsaltsolutionafterthreedays，removecellsonthebottomforevery3daysoftime，tobecovered而mculturedcellsatthebottomofbottlesafterusingtrypsintodigest，andthenjointheserummediumforterminationofdigestion,jointheappropriatemediumcount，theninoc

5、ulateinthenewculturebottle．CountingoftherequiredamountofMSCs(morethan2X107)，putthecellsintoliquidnitrogentopreserve．2、animalexperimentWistarmts30，male，200garound，randomlydividedintoA，B，C3groups，each、析血10．Anesthetizebyketamineinabdominal，revealleRlegsciaticnervei

6、nsterileconditions，cutapartthenerveinthepiriformismarginofabout3mm，whichisthesciaticnervestemabout6mm，retractastheirnatural．Operationwillbeunderthemicroscopeoftherespectiveendsofthesiliconesetsofpeoplewithin1．0mln，8／0non-invasivesutureclosureoftheoutermembranean

7、dsiliconewallSOthatmakingthetwoendsofthesiliconetubefrom10mm．GroupA：putMSCs+bFGFsolution(concentrationofabout2×107／m1)intosiliconetube；BGroup：putMSCssolution(concentrationofabout2×107／m1)intosiliconetube；CGroup：siliconetubefilledwithnormalsaline；threegroupswereu

8、singfibringlueclosedinterface，suturewounds．analysisofresults1、generalobservation：MSCs+bFGFgroup，onlyoneratlimbsfoothasulcersandedema,mostoftheothertwogroupsareemergin