脑心通对大鼠局灶性脑缺血再灌注损伤的保护作用

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1、山东大学硕士学位论文组明显高于A组，C组明显低于B组。三组阳性细胞率比较有显著差异。2．WesternBlot法显示：脑缺血灶范围内TNF-a、IL-IB的表达，B组明显高于A组，C组明显低于B组。研究结论：脑心通通过抑制脑缺血再灌注损伤后小胶质细胞的活化及TNF-Q、IL-IB的表达，抑制炎症反应，对脑缺血大鼠有一定程度的神经保护作用。研究意义。本篇论文进一步证实了炎症反应是脑缺血再灌注时组织持续损伤的重要特征之一，基本阐明急性脑缺血与小胶质细胞激活、炎症介质释放的关系。证实脑心通对脑缺血再灌注后

2、的炎症的抑制作用，为急性脑缺血炎症性损伤提供了新的有效干预措施。关键词：缺氧缺血，脑；肿瘤坏死因子；自细胞介素l；炎症；大鼠，sprague_dawley2山东大学硕士学位论文．一一_-_—_—-_————二———————————————————————————————。——。—————一英文摘要TheprotectiveeffectofthereperfusionafterfocalcerebralischemiaofratsbyNaoxintongMasterQl也iXiIlTutorDuYif

3、engDepartmentofNeurology，TheShandongProvincialHospital，ShandongUniversity,Jinan250021，ShandongAbstractTheinflammationfollowingcerebralischemia-repcrfusionplaysanimportantroleinthebrain埘u巧．Peoplepaysmoreandmoreattentiontoittheseyears．Microglia,oneofthen

4、eurogliacells，ismainimmunologicalcellinthebrain,canreleaseinflammatoryfactorssuchasturnernecrosisfactor-a(TNF-a)，interleukin-1p(IL-16)whenactivated．Thesefactorsplaycriticalroleintheinflammatory．Objective：Toinvestigatetheeffectoftheactivationofmicroglia

5、andtheexpressionof，n盯·Q，IL-1{3therepcrfusionafterfocalcerebralischemiaofratsbyNaoxintong．Exploree胁ivememodtointerferetheinflammationfollowingcerebralischenfia-rcperfusioninjury．Methods：1．ThirtymaleSDratsWererandomlydividedintothre炮groups：shamoperatedgr

6、oup(Agroup，n=10)，Ischemicgroup(Bgroup，n=10)，Naoxintonggroup(Cgroup，n=10)．2．AccordingtotheinstructionofNaoxintong，dissolvethemedicamentalpulve均：taofNaoxintongcollocystiswithdistilledwater．Takeclinicalmedicationofhumanxexluivalemdoseratioofanimalasrefere

7、nceconcentration．O．149／kg．dintragastricadministrationtoCgroupfor2weeks．AandBgroupswereintragastricedwithequaldistilledwater．Tookoperationlhourafterthelastintragastricadministration．3immunohistochemistryandWesternBlot．Results：1．Immunohistochemistory：the

8、expressionofCD68、IL·lpandTNF-aweremarkedlyincreasedinBgroupthanthoseinAgroup；TheexpressionofCD68、IL·1BandTNF-aweresignificantlylowerinCgroupthanthoseinBgroup．2．Westernblot：theexpressionofIL-IIBandTNF-也weremarkedlyincreasedinBgroupthanth