阻断shh信号通路对胶质细胞炎性激活的实验研究

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1、广州医学院硕士研究生学位论文阻断SHH信号通路对胶质细胞炎性激活的实验研究EffectofblockingtheSHHsignalingpathwayoninflammation-activedofgliomacell专业名称：人体解剖与组织胚胎学学位申请人：王迪学号：2009211957指导老师：洪乐鹏副教授广州医学院广州二0一二年三月目录摘要.............................................................................................................

2、...................1Abstract..........................................................................................................................4中英文对照表......................................................................................................7前言.....................

3、.......................................................................................................8第一部分LN229细胞炎症激活模型的建立及阻断SHH通路对炎症因子表达的影响..............................................................................................................................111实验材料........

4、................................................................................................111.1实验细胞........................................................................................................111.2实验主要试剂...............................................................

5、.................................111.3实验仪器........................................................................................................111.4主要试剂的配制............................................................................................121.5PCR引物.......................

6、................................................................................122实验方法........................................................................................................132.1细胞培养...................................................................................

7、.....................132.2细胞处理及分组............................................................................................142.3CCK-8法检测细胞存活...............................................................................142.4实时荧光定量PCR................................................

8、...................