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1、HPLC测定人血清苯巴比妥、卡马西平、苯妥英钠浓度及其与FPIA法测定结果的比较12*2222程振田,郭瑞臣,王本杰,魏春敏,袁桂艳,孔祥麟(11潍坊市益都中心医院,山东青州262500;21山东大学齐鲁医院临床药理研究所,济南250012)摘要:目的建立能同时测定人血清苯巴比妥(PB)、卡马西平(CBZ)、苯妥英钠(PT)3种药物浓度的高效液相色谱(HPLC),并与常规荧光偏振免疫法(FPIA)测定结果进行相关性分析。方法以乙酸乙酯-二氯甲烷(4B1)为提取溶剂,固定相Diamon--1silC18柱(416mm@250mm,5Lm),流动相为乙腈-
2、水(40B60),紫外检测波长285nm,流速110mL#min。收集癫痫患者服药后稳态谷浓度血样,分别用HPLC和FPIA法测定,考察2种方法的相关程度。结果PB,CBZ,PT的标准曲线范围分别为-1-1110~4510,011~1510,110~2510mg#L,最低检测浓度分别为015,0105,015mg#L,提取回收率分别为8012%,7812%,8612%,日内和日间RSD均小于9%。PB、PT2种方法的测定值无统计学差异(P>0105),但CBZ测定值FPIA法高于HPLC(P<0101)。结论HPLC操作简单、灵敏、准确,适用于治疗药物
3、监测;PB和PT2种测定方法有良好的相关性,CBZ不同测定方法,结果存在差异。关键词:苯巴比妥;卡马西平;苯妥英钠;高效液相色谱法;荧光偏振免疫法中图分类号:R91714;R44616文献标识码:A文章编号:1001-2494(2008)12-0950-05DeterminationandComparisonofPhenobarbital,CarbamazepineandPhenytoininHumanSerumbyHPLCandFPIAMethods12*2222CHENGZhen-tian,GUORu-ichen,WANGBen-jie,WEICh
4、un-min,YUANGu-iyan,KONGXiang-lin(11YiduCentralHospitalofWeifang,Qingzhou262500,China;21InstituteofClinicalPharmacology,QiluHospitalofShandongU-niversity,Jinan250012,China)ABSTRACT:OBJECTIVEToestablishaHPLCmethodforsimultaneousdeterminationofphenobarbital(PB),carbamazepine(CBZ)an
5、dphenytoin(PT)concentrationsinhumanserum,andtoevaluateitsrelationshiptoconcentrationsdeterminedbyFluores-cenePolarizationImmunoassay(FPIA)method1METHODSSerumsampleswerecollectedfromwellcontrolledepilepsypatientstrea-tedwithPB,CBZandPTinQiluHospitalofShandongUniversity,anddetermi
6、nedbyHPLCandFPIAmethodsrespectively1Thesam-pleswereextractedwithacetoacetate:dichlormethane(4B1),separatedonaC18reversed-phasecolumn(416mm@250mm,5Lm),e-lutedwithmobilephaseof40B60acetonitri-lwater,anddetectedat285nm1RESULTSThecalibrationcurveswerelinearwithinthe-1-1-1rangeof110-
7、4510mg#LforPB,011-1510mg#LforCBZ,110-2510mg#LforPT,thelimitsofdeterminationwere-1015,0105,015mg#L,andtheaveragerecoverieswere8012%,7812%,8612%respectively1Theinter-andintra-dayRSDswerelessthan9%1NosignificantstatisticdifferencesexistedbetweenFPIAandHPLCmethodforPBandPT(P>0105),b
8、utforCBZ,theconcentrationdeterminedbyFPIAwerehi
