ghrelin对大鼠离体胰腺胰岛素分泌与glut-2表达的影响

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1、面积及平均光密度均显著低于对照组。高脂肥胖组中Ghrelin组对照单纯高糖灌注组Glut．2染色阳性面积及平均光密度均显著降低。五、透射电镜观察各组胰腺形态改变高脂肥胖组呈不同程度的调亡征象：D细胞核膜扩张，细胞线数量减少，部分肿胀，线粒体嵴和基质溶解，部分形成空泡。内质网扩张；完整的分泌颗粒数量显著减少，且多数已失去了正常的形态特征。高尔基体囊泡扩张，细胞质内结构密度增大；少数细胞可见核固缩，染色质边集，胞浆浓缩，内质网疏松并与胞膜融合形成空泡，高脂肥胖组中Ghrelin组细胞器损害程度明显减

2、轻：p细胞内有大小不等被由单层界膜包绕的空泡，含致密核芯的分泌颗粒数量减少。部分p细胞核轻度变性，胞浆内也存在少量的空泡变性，核周间隙扩张。线粒体结构大致正常，高尔基体轻度扩张。结论l、在大鼠离体胰腺中，Ghrelin可促进高浓度葡萄糖诱导的胰岛素分泌。2、Ghrelin可以减轻高糖诱导胰岛D细胞的凋亡，起保护作用。3、Ghrelin可以降低Glut．2在胰腺p细胞中的表达。关键词Ghrelin；离体胰腺灌注；胰岛；Glut-23英文论著摘要Thee们’ectofghrelinoninsulin

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