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1、ActaPhysiologicaSinica,August25,2005,57(4):433-438433http://www.actaps.com.cnResearchPaperExpressionofman,aforminhomologydomain-containinggeneinthemouselimbCUIYan-Zhen1,*,PENGYi-Feng2,TIANQi31DepartmentofEnvironmentalandBiomolecularSystems,OGISchoolofScienceandEngineering,OregonHealthand
2、ScienceUniversity,20000NWWalkerRd.,Beaverton,OR97006,USA;2InstituteofReproductiveMedicine,YijishanHospital,WannanMedicalCollege,Wuhu241001,China;3DepartmentofPathology,L113,SchoolofMedicine,OregonHealthandScienceUniversity,3181SWSamJacksonParkRoad,Portland,OR97239,USAAbstract:Formindefin
3、esafamilyofstructurallyrelatedproteinsthathavetwoforminhomologydomains(FH1andFH2).Variousmutationsintheforminlocusresultinlimbdeformity,suggestingthatthesegenesplayindispensablerolesinthelimbdevelopment.HerewereporttheisolationofanovelcDNA,man,fromthemouselimb,whichcontainstwoconservedFH
4、1andFH2domains.Itsexpressionisdescribedandpossiblefunctionalsignificanceisdiscussed.Keywords:man;formin;FH1;FH2;mouselimb;cytokinesis;expressioncloning基因“慢”的克隆及其在小鼠组织中的表达崔衍贞1,*，彭弋峰2，田启31俄勒冈医科大学环境与生命科学系，俄勒冈州97006，美国；2皖南医学院弋矶山医院生殖医学研究所，芜湖241001；3美国俄勒冈医科大学病理学系，俄勒冈州97239，美国摘要：Formin蛋白家族由结构相关
5、的蛋白组成，它们都有两个formin同源功能区(forminhomologydomains)，FH1和FH2。这些基因上的多种变异均导致动物四肢畸形，提示这些基因在肢体发育中起重要作用。我们自小鼠肢体基因库中分离出了一个新基因：“慢”，该基因含有FH1和FH2。我们检测了该基因在小鼠胚胎及成体的表达情况，并对可能的功能意义进行了讨论。关键词：man基因；formin蛋白；formin同源功能区：FH1和FH2；鼠四肢；细胞分裂；表达克隆中图分类号：Q956Thefirsttwoallelesofthemouselimbdeformity(ld)mains(FH1andF
6、H2),althoughsomemembersinthismutation,whichwereisolatedinthe1960s,areamongfamilycontainathirdhomologydomainFH3[5].thoseclassicalmousemutationsthatdisruptlimbmorpho-Forminrelatedproteinsaregenerallythoughttoplaycru-genesis[1].Forminwasproposedtobetherelevantgenecialrolesincytoskeletalproc
7、esses,especiallyincytokine-becauseinthreeofthefiveldallelestheC-terminaldo-sisandestablishmentofcellpolarity[6].Consistentwiththis,mainwasdisrupted[2].Overthelasttenyears,afamilyofinteractionsoftheseproteinswithactincablesandtheirformin-relatedgeneshavebeenisolated,includ
