资源描述：
《斑茅cdna中抗病基因同源序列的分离和表达特性分析》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。
1、作物学报ACTAAGRONOMICASINICA2009,35(11):2022−2028http://www.chinacrops.org/zwxb/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2009.02022IsolationandCharacterizationofDiseaseResistanceGeneAnalogsfromErianthusarundinaceuscDNA**QUEYou-Xiong,XU
2、Li-Ping,LINJian-Wei,XUJing-Sheng,ZHANGMu-Qing,andCHENRu-KaiKeyLaboratoryofSugarcaneGeneticImprovement,MinistryofAgriculture/FujianAgricultureandForestryUniversity,Fuzhou350002,ChinaAbstract:Plantdiseaseresistancegenes(R-genes)encodesomeconservedmotifs.Accordingtotheco
3、nservedmotifspresentintheknownNBS-LRRR-genesequencesandRgeneanalogs(RGAs),severaldegenerateprimersweredesignedandappliedintheRGAisolationfromErianthusarundinaceususingPCRapproach.Intotal,6RGAsweresuccessfullyobtained,withGenBankaccessionnumbersofEU685835,EU685836,EU68
4、5837,EU685838,EU685839,andEU685840.MultiplealignmentsshowedthattheencodingsequencesofthesixcloneswerehighlyconservedandstrikinglysimilartotheelevenmosttypicalNBS-LRRtypeR-genepeptidesequences,especiallyatthefourNBSmotifsofP-loop,kinase-2,kinase-3a,andHD.Theidentityper
5、centageattheamino-acidlevelrangedfrom8.3%to93.0%amongall17sequencestestedandfrom30.5%to45.6%amongthesixRGAsclonedinthisstudy.Theresultsofclusteranalysisandtheexistenceofanasparticacidresidue(D)atthefinalresiduepositionofthekinase-2motifalsoindicatedthatallofE.arundina
6、ceusRGAsmightbelongtonon-TIRgroup.TheReal-timePCRresultsshowedthatalloftheRGAswereconstitutivelyexpressedinroots,stalksandleavesofE.arundinaceus,andtheirexpressioncouldbeup-regulatedinleavesbytheexogenoussignalmoleculesSAandH2O2.Therefore,itsuggestedthatE.arundinaceus
7、RGAsmightplayimportantrolesindiseaseresistanceinanSA-andH2O2-dependentdefenseresponsepathway.Furtherstudiesshouldaimtoclonefull-lengthR-genesinE.arundinaceusandcharacterizetheirfunctionsindefenseresponses.Keywords:Erianthusarundinaceus;Resistancegeneanalogs(RGAs);Dege
8、nerateprimers;Real-timePCR斑茅cDNA中抗病基因同源序列的分离和表达特性分析**阙友雄许莉萍林剑伟徐景升张木清陈如凯福建农林大学/农业部甘蔗遗传改良重点开放实验室,福建福州350002摘要:植物抗病基因具有一些特定的保守结
