毕业论文--葡萄逆境胁迫诱导型启动子的克隆

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1、青岛农业大学毕业论文题目:葡萄逆境胁迫诱导型启动子的克隆姓名:刘景祎学院:生命科学学院专业：生物技术班级：2006级2班学号：20062051指导教师：薛仁镐完成时间：2010.6.142010年6月17日目录中文摘要···································································································1Abstract··················································································

2、···················21．引言···································································································32．材料与方法··························································································62.1材料········································································

3、····························62.2方法····································································································62.2.1引物序列的设计···················································································62.2.2组培培育葡萄幼苗·······················································

4、·························62.2.3DNA的提取························································································62.2.4PCR扩增····························································································72.2.5凝胶电泳检测···························································

5、··························82.2.6基因片段回收·····················································································82.2.7与T载体连接及产物转化······································································82.2.8质粒的提取··············································································

6、··········92.3.9酶切鉴定···························································································93．结果与分析···························································································93.1DNA提取鉴定··············································································

7、·········93.2PCR扩增结果·······················································································103.3质粒提取鉴定······················································································10