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1、现代食品科技ModernFoodScienceandTechnology2014,Vol.30,No.9传统酸面团中细菌与酵母菌的分离与鉴定1111,2111刘同杰,李云,吴诗榕,金乐天,张国华,杨浣漪,何国庆(1.浙江大学生物系统工程与食品科学学院,浙江省食品微生物技术重点实验室,浙江大学馥莉食品研究院,浙江杭州310058)(2.朝鲜韩德秀平壤轻工业大学,朝鲜平壤)摘要:为进一步描述我国传统面食发酵剂的理化性质和菌落组成,收集了北方地区6份发酵剂样品,测定了其酸度和菌落总数,并对分离、纯化、初筛后得到的75株细菌和60株
2、酵母菌进行了测序鉴定。结果显示,样品pH值范围为3.73~5.46,总滴定酸度为8.3~19.8mL;乳酸菌和酵母菌的计数结果分别为8.35±0.07~9.75±0.12Logcfu/g,6.31±0.22~8.68±0.04Logcfu/g。鉴定出包括短乳杆菌(Lactobacillusbrevis)、植物乳杆菌(Lactobacillusplantarum)和旧金山乳杆菌(Lactobacillussanfranciscensis)在内的乳酸菌8种;酵母菌4种,其中优势菌为酿酒酵母(Saccharomycescerevi
3、siae);其他细菌6种,主要为解淀粉芽孢杆菌(B.amyloliquefaciens)、地衣芽孢杆菌(B.licheniformis)和醋杆菌。结果表明,我国传统面食发酵剂菌相复杂,以酵母菌和乳酸菌为主,还包括芽孢杆菌、醋酸杆菌在内的多种其他细菌,甚至可能含有致病菌。通过比较细菌和酵母在不同酸面团样品中的分布,发现不同来源样品的微生物种类组成存在差异。关键词:酸面团;菌相分析;16S/26SrDNA测序;生物多样性;系统发育树文章篇号:1673-9078(2014)9-114-120DOI:10.13982/j.mfst.
4、1673-9078.2014.09.020IsolationandIdentificationofBacteriaandYeastfromChineseTraditionalSourdoughLIUTong-jie1,LIYun1,WUShi-rong1,JINLe-tian1,2,ZHANGGuo-hua1,YANGHuan-yi1,HEGuo-qing1(1.CollegeofBiosystemsEngineeringandFoodScienceofZhejiangUniversity,Zhejiangprovincia
5、lkeylaboratoryofFoodMicrobiology,FuliInstituteofFoodScienceofZhejiangUniversity,Hangzhou310058,China)(2.DPRKHanDexiuPyongyangUniversityoflightindustry,Pyongyang,DPRK)Abstract:Inthisstudy,thephysicochemicalpropertiesandmicrobialprofileoftraditionallyfermentedsourdou
6、ghforChinesesteamedbreadwereexamined.SixsamplesofsourdoughwerecollectedfromnorthernChina.Acidityandcolonycountsweremeasured.Afterisolation,purification,andpreliminaryscreening,75strainsofbacteriaand60strainsofyeastswereobtainedandidentifiedbyDNAsequencing.ThepHwasb
7、etween3.73and5.46andtotaltitratableacid(TTA)valuesrangedfrom8.3to19.8mL.Inallthesamples,thenumberoflacticacidbacteria(LAB)andyeastsrangedfrom8.35±0.07to9.75±0.12Logcfu/gand6.31±0.22to8.68±0.04Logcfu/g,respectively.EightLABspecies,includingLactobacillusbrevis,L.plan
8、tarum,andL.sanfranciscensis,andsixotherbacterialspecies,includingBacillusamyloliquefaciens,Bacilluslicheniformis,andthreespeciesofAcetobacter,wer