basic study of retinal stem cell separation from mo...

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1、MedMolMorphol(2010)43:139144©TheJapaneseSocietyforClinicalMolecularMorphology2010DOI10.1007/s00795-009-0486-3ORIGINALPAPERNaokiYamamoto·AtsuhiroTanikawaMasayukiHoriguchiBasicstudyofretinalstem/progenitorcellseparationfrommouseiristissueReceived:October6,2009/Accepted:November17,2009A

2、bstractWedescribedthepossibilityofretinalregenera-thisregenerationprocess,tissuestem(multipotentstem)/tionusinganovelandefficienttechniqueforculturingandprogenitorcellsfoundinvarioustissuesinadultshaveseparatingretinalstem/progenitorcellsfromiristissue.attractedattentionandhavebeenuse

3、dtogeneratemam-37Immunohistochemicalstainingofadultagoutimouseirismalianretina.Theuseofeyeballtissues,includingimma-8,9101113tissuerevealedthepresenceofnestin/low-affinityneuro-tureretinaltissue,theciliarybody,andtheiris,hasNTRtrophinreceptorp75(p75)-positivecellsontheendothe-alsobeen

4、reported.Consideringtheirpossibleclinicalappli-liumcameraeanteriorisside.Culturedmouseiris-derivedcation,wehavebeeninvestigatingretinalregenerationcellscontainedlittleornomelaninandwerefoundtobeusingautologoustissuesandhavefocusedoniristissue,positivefornestin.Mostnestin-positivecell

5、swereanalyzedwhichembryologicallybelongstotheopticcupinnerlayer,NTRforthecoexpressionofp75asacellmembraneprotein.asdoestheneuralretina,andallowsthecollectionofautol-NTRWhenthep75wasusedasamarkertosortthecells,weogoustissueinasafemanner.Wedescribeanoveltech-obtainedadensepopulationofn

6、estin-positivecells.Fur-niquedevelopedinourlaboratorythatusesacellmarkerNTRNTRthermore,thenestin/p75-positivecellswereabletodif-ofthelow-affinityneurotrophinreceptorp75(p75;p75ferentiateintoneuralretinacells.Thus,thiscultureandnervegrowthfactorreceptor;CD271)toobtainretinalseparationt

7、echniqueisusefulforobtainingretinalstem/stem/progenitorcellsthatcandifferentiateintoneuralprogenitorcellsfromadultmouseiristissueandfortheretinacells.efficientproductionofneuralretinacells.KeywordsRetinalstem/progenitorcells·Low-affinityneu-NTRMaterialsandmethodsrotrophinreceptorp75(p7

8、5)·Nestin·Ir