hydrogel microchannel for cell adhension

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1、ViewOnline/JournalHomepageLabonaChipDynamicArticleLinksCitethis:DOI:10.1039/c2lc40611jwww.rsc.org/locTECHNICALINNOVATIONCircular,nanostructuredandbiofunctionalizedhydrogelmicrochannelsfordynamiccelladhesionstudies{abccabSebastianKruss,*LuiseErpenbeck,MichaelP.Scho¨nandJoachimP.Spa

2、tzReceived28thMay2012,Accepted13thJuly2012DOI:10.1039/c2lc40611jWereportonamethodtofabricatebiofunctionalizedpolyethyleneglycolhydrogelmicrochannelswithadjustablecircularcross-sections.TheinnerchannelsurfacesaredecoratedwithAu-nanoparticlearraysoftunabledensity.TheseAu-nanoparticl

3、esarefunctionalizedwithbiomoleculeswhereasthehydrogelmaterialprovidesaninertandbiocompatiblebackground.Thistechnologyprovidescontroloverflowconditions,channelcurvatureandbiomoleculedensityonthechannelsurface.Itcanbeappliedforbiophysicalstudiesofcell-surfaceinteractionsmimicking,fo

4、rexample,leukocyteinteractionswiththeendotheliallininginsmallvessels.10Introductionmajorchallenge.Especiallyforbiologicalapplications,cova-lentandsite-directedimmobilizationofbiomoleculesisdesir-Overthepastdecade,microfluidicshasbecomeavaluabletoolforable.Closelyrelatedtosurfacefu

5、nctionalizationisthequestioncellbiologybecauseitcanprovidecontroloversmallfluidofhowmanymoleculesasurfacemustpresenttomimica1volumesandbiophysicalparameterssuchasshearstress.Sofar,biologicalinterfaceorperformaspecifictask.Thedensityandmostoftheapproachesusedtofabricatemicrostructu

6、redsystemsspatialpresentationofadhesionsitesonsurfacescanhavea2forcelladhesionstudiesarebasedonsoftlithography.Withtop-dramaticinfluenceoncelladhesion.11Asaprominentexample,downmethods,thecross-sectionisusuallylimitedtoarectangularadhesiveinteractionsofcirculatingbloodcellswiththe

7、geometry.However,thetopographyandthree-dimensionalshapeendotheliumduringinflammationaregovernedbytheup-andDownloadedbyColumbiaUniversityon06August2012ofthesubstratemustbetakenintoaccountwheninvestigatingdownregulationofcellsurfacereceptordensity.12cellularinteractionsascellshavebe

8、enshowntorespondtotheTheuseofPDMS