分子印迹固相微萃取抗生药

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1、AnalBioanalChem(2012)403:785–796DOI10.1007/s00216-012-5901-2ORIGINALPAPERDevelopmentofnovelmolecularlyimprintedsolid-phasemicroextractionfibersandtheirapplicationforthedeterminationofantibioticdrugsinbiologicalnsamplesbySPME-LC/MSMalgorzataSzultka&JacekSzeliga&MarekJack

2、owski&BoguslawBuszewskiReceived:22November2011/Revised:8February2012/Accepted:24February2012/Publishedonline:13March2012#TheAuthor(s)2012.ThisarticleispublishedwithopenaccessatSpringerlink.comAbstractNovelmolecularlyimprintedpolymer(MIP)-KeywordsSolid-phasemicroextracti

3、on.Antibioticdrugs.coatedfibersforsolid-phasemicroextraction(SPME)fibersHigh-performanceliquidchromatography.Masswerepreparedbyusinglinezolidasthetemplatemolecule.spectrometryThecharacteristicsandapplicationofthesefiberswereinvestigated.Thepolypyrrole,polythiophene,andp

4、oly(3-methylthiophene)coatingswerepreparedintheelectro-Introductionchemicalpolymerizationway.ThemolecularlyimprintedSPMEcoatingsdisplayahighselectivitytowardlinezolid.Determinationofchemicalcompoundsfromvariousmatri-Molecularlyimprintedcoatingsshowedastableandrepro-ces,

5、includingenvironmentalandbiological(blood,plasma,ducibleresponsewithoutanyinfluenceofinterferentscom-urine),isaseriousprobleminmodernanalyticalchemistry.monlyexistinginbiologicalsamples.High-performanceChemicalcompoundsanalyzedattheppmandppblevelsliquidchromatographywit

6、hspectroscopicUVandmassrequiretheapplicationofadequateanalyticalprocedures.spectrometry(MS)detectorswereusedforthedetermina-Themostimportantstageintraceanalysesisundoubtedlytionofselectedantibioticdrugs(linezolid,daptomycin,samplepreparationmethods.Suchsamplingmethodsas

7、theamoxicillin).Theisolationandpreconcentrationofselectedliquid–liquidextractionorsolid-phaseextraction(SPE)antibioticdrugsfromnewtypesofbiologicalsamples(acel-havebeenknownformanyyears.Samplescollectionmeth-lularandprotein-freesimulatedbodyfluid)andhumanods,duetotheiri

8、mportance,arefrequentlycalledabottle-plasmasampleswereperformed.TheSPMEMIP-coatedneckoftheentireanalyticalproc