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1、・1・本科毕业论文(设计)题目：花生茎水溶性膳食纤维(SDF)的提取工艺研究花生茎水溶性膳食纤维(SDF)的提取工艺研究摘要釆用单•因素和正交实验探讨了解法提取花生杆中水溶性膳食纤维(SDF)的提取工艺。木瓜蛋口酶预处理的花生杆粉的最佳提取工艺条件为：纤维素酶浓度为1.0mg/ml,温度为40°C,pH值为4.8,水解时间为1小时。釆用上述最佳提取工艺制备SDF,得到的样品的酶解率为13.4%,聚合度为1.0036,SDF的得率为1.25%。屮性蛋白酶预处理的花生杆粉的最佳提取工艺条件为：纤维素酶浓度为0.5mg/ml,水解温度为40°C,pH值为4.4,水解时间为0.5小
2、时。采用上述的最佳工艺条件制备SDF,得到的样品的酶解率为14.10%,聚合度为1.1435,SDF的得率为2.23%。论文还研究了SDF的抗氧化活性。实验结呆表明，两种蛋口酶预处理的花生杆粉制备的SDF均具有清除径自由基、超氧阴离子自由基、DPPH自由基、铁述原能力和钳述原能力的抗氧化活性。关键词木瓜蛋白酶；中性蛋白酶；纤维索酶；水溶性膳食纤维；抗氧化活性-3-StudyonthetechnologyofextractingsolubledietaryfibrefrompeanutpowderAbstractSinglefactorandorthogonaldesigno
3、fenzymeextractedunderpeanutsinwater-solubledietaryfiber(SDF)oftheextractionprocess.Papainpretreatmentofpeanutpowderundertheoptimumextractionconditionsareasfollows:theconcentrationof1.0Cellulasemg/ml,temperature40°C,pHvalueof4.8,thehydrolysistimeis1hour.Thebestextractionprocessusingtheabove
4、-mentionedPreparationSDF,theenzymesamplesobtainedwas13.4%forthedegreeofpolymerizationof1.0036,SDFsarateof1.25percent.Pretreatmentneutrasepeanutpowderundertheoptimumextractionconditionsareasfollows:theconcentrationof0.5Cellulasemg/ml,thehydrolysistemperatureof40°C,pHvalueof4.4,thehydrolysis
5、timeof0.5hours・Thebestuseoftheabove-mentionedpreparationprocessconditionsSDF,theenzymesamplesobtainedwas14.10%,polymerizationdegreeto1・1435,SDFsarateof2.23percent.PaperalsoexaminedtheantioxidantactivityoftheSDF.TheexperimentalresultsshowthatproteasepretreatmentofthetwopeanutpowderundertheS
6、DFarescavenging,superoxideanionradical,abilitytodietaryDPPHfreeradical,ironandmolybdenumtorestoretherestoretheabilityofantioxidantactivity.Keywords:papain;neutralprotease;Cellulase;water-solublefiber;antioxidantactivity目录1引言??????????????????????????????？11」膳食纤维的研究意义??????????????????????1
7、.2膳食纤维的生理功能??????????????????????121控制J体重？??？??？??？??？???????????？1.2.2防治便秘、调节肠道???????????????????？1.2.3防治结肠癌???????????????????????？1.2.4防治糖尿病、高血压、心脏病和动脉硬化???????????1.2.5调节血脂,促进新陈代谢??????????????????1.2.6清除外源有害物质????????????????????？127其彳也生理功能？?？？??？??？??？?
