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1、叶绿酸铜钠对结直肠肿瘤抑制作用实验探［摘要］目的：研究叶绿酸铜钠(CHL)对DMH(1,2-二甲基腓二盐酸盐)诱导的小鼠结直肠肿瘤的抑制作用。方法：80只幼鼠(4〜5周)随机分为阴性对照组(NC组)、阳性对照组(DMH组)、CHLA组和CHLB组。DMH组和CHL组背部皮下注射DMH诱癌；NC组背部皮下注射生理盐水作对照。以CHL灌胃处理CHLA和CHLB组动物。处死小鼠后，解剖小鼠结直肠，固定，观察肿瘤发生情况，免疫组化染色示PCNA、Ras-p21及caspase-3等细胞增殖及凋亡相关因子的表达情况。结果:两CHL干预组小鼠结直肠肿瘤发生率与DMH
2、组相比，差异无统计学意义(P>0.05)；但其平均每鼠荷瘤数及结直肠癌百分率都显著低于DMH组(P0.05)；两CHL干预组小鼠肠黏膜PCNA、Ras-p21蛋白表达水平均显著低于DMH组(P0.05)□结论：CHL在诱癌起始阶段和始发后阶段给药均可抑制DMH诱导的小鼠结直肠肿瘤的发生，降低每鼠平均荷瘤数及肠癌百分率，这可能与其下调结直肠黏膜PCNA,Ras-p21水平，从而间接调控细胞增殖和凋亡平衡有关，CHL有望成为预防结直肠癌的理想化学制剂。［关键词］叶绿酸铜钠；小鼠；结直肠肿瘤［中图分类号］R735E文献标识码］A［文章编号]1673-7210(
3、2008)07(b)-020-03RestTainingeffectofSodiumCopperChlorophyllinoncolorectalcancerofmiceWANGQing,WANGXin-ming(DepartmentofAnatomy,WeifangMedicalCollege,Weifang261042,China)[Abstract]Objective：Toresearchpossiblerestrainingeffectofsodiumcopperchlorophyllin(CHL)onDMH-inducedcolorectait
4、umourinmice・Methods：80mice(male)(4〜5weeks)wererandomlydividedintonegativecontrolgroup(NCgroup,inwhichthemiceweregivenhypodermicinjectionofphysiologicalsaline),DMHgroup,CHLAgroupandCHLBgroup(themiceinthesethreegroupsweregivenhypodermicinjectionofDMHontheback,thoseinCHLgroupweregiv
5、enCHLintragastrieadministration).Thewholecolonandrectumweredissectedtoobserveshapeanddistributionoflargeboweltumour,markedwithnumber,fixedandimmunohistochemistrystains・ResuIts：TherewasnosignificantdifferencebetweenincidenceofcolorectaicarcinomaofCHLgroupandDMHgroup(P>0.05).Whilei
6、ncidenceofcolorectaicancer,averagenumberoftumour,andthepercentageofcarcinomainthesetumoursofCHLgroupwereallsignificantlylowerthanthoseofDMNgroup(P0.05).Infurthermechanismexploration,PCNAandRas~p21expressionlevelofCHLtreatedgroupswerebothfoundtobeelevatedthanthoseofDMHgroup(P0.05)
7、treatment・Conclusion：CHLcansuppresstheincidenceofDMH-inducedcolorectaitumourinmice,whetheritisgivenattheinitiationorinthemiddleofDMHtreatmerit.ThiseffectmaybepartlyattributedtoCHL"sreducingeffectonPCNAandRas~p21expressionandthesubsequentsuppressioneffectonexcessivecellproliferati
8、crn..Keywords]SodiumCopperChlorophyllin；
