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1、JournalofNeuroirnmunology,14(1987)305-316305ElsevierJNI00477AnalysisoftheintrathecalhumoralimmuneresponseinBrownNorway(BN)rats,infectedwiththemurinecoronavirusJHMR.DiSrries,R.Watanabe,H.WegeandV.terMeulenInstitutfftrVirologieundImmunbiologie,UnioersitiitW'~rzburg~VersbacherStr.7,D-8700llr~rzburg
2、F.R.G.(Received19August1986)(Accepted16October1986)Keywords:(BrownNorwayrat);CoronavirusJHM;Demyelination;Antibodysynthesis;(Intrathecal);IsoelectricfocusingSummarySerumandCSFspecimensfromclinicallyhealthyBrownNorway(BN)ratsinoculatedintracerebrallywithcoronavirusJHMwereanalysedwithrespecttothes
3、tateoftheblood-brainbarrier(BBB)andtheintrathecalsynthesisandisoelectricdistributionofimmunoglobulins(Ig).IncreasedCSF/serumratiosforIginthecontextofanintactBBBwereneverseenintheabsenceofintrathecalsynthesisofvirus-specificantibodies.Affinity-mediatedimmunoblotanalysisrevealedabroadpatternofviru
4、s-specificantibodieswithembeddedclustersofrestrictedheterogene-ity,butnosignsofoligoclonalIgproductioncarryingnon-viralspecificity.FromthesedataitwasconcludedthatBNratsdocontroltheintracerebralspreadofJHMviruseffectivelybyastronglocalvirus-specificantibodyresponse,therebyprevent-ingaclinicallyap
5、parentdisease.IntroductionThemurinehepatitisvirusMHV4,strainJHM,isknowntocauseasubacutedemyelinatingencephalomyelitis(SDE)inratsafteranincubationperiodofweeksAddressforcorrespondence:R.Di~rries,Institutf'tirVirologieundImmunbiologie,Universit~itWiirzburg,VersbacherStr.7,D-8700Wiirzburg,F.R.G.016
6、5-5728/87/$03.50©1987ElsevierSciencePublishersB.V.(BiomedicalDivision)306ormonths(Nagashimaetal.1978,1979;Sorensenetal.1980;Wegeetal.1982).Inoneofthemostsusceptibleratstrains,theLewisrat,SDEisclinicallycharacterizedbyataxicgaitandhindlegparalysis.Manyanimalssurvivethisdiseasebutmaylaterdevelopar
7、elapseofthisdisorder(Wegeetal.1984a).Histologicallyperivascu-larcuffingswithwell-defineddemyelinatedplaquesareseenpredominantlylocatedinthebrain-stemandspinalcordwithviralantigenpresentingliacellsintheneighbourhoodofplaques.
