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1、VIROLOGY130,527532(1983)SynthesisandSubcellularLocalizationoftheMurineCoronavirusNucleocapsidProteinSTEPHENA.STOHLMAN,'JOHNO.FLEMING,CHRISD.PATTON,ANDMICHAELM.C.LAIDepartmentsofNeurologyandMicrobiology,UniversityofSouthernCal$brnia,SchoolofMedicine,2025.&ma1Avenue,LosAn
2、geles,California900.3.9ReceivedJune9,1983;acceptedAugust3,1985Thesynthesisandprocessingofthenucleocapsidprotein(~~60)oftheJHMstrainofmurinecoronaviruseswereexamined.Pulse-chaseexperimentsshowedthatpp60wassynthesizedinitiallyasaproteinofapproximately57,000inmolecularweig
3、ht(~57).Immunoprecipitationusingmouseanti-JHMVantiserumindicatedthat~57wasvirusspecific.Immunoprecipitationwithmonoclonalantibodiesspecificforpp60showedthatp57wasantigenieallyrelatedtopp60andwasnotphosphorylated,whiletheintracellularproteinthatcomigratedwiththevirionnuc
4、leocapsidprotein,pp60,wasphosphorylated.The~57wasfoundexclusivelyinthecytosolwhilethemajorityofpp60wasassociatedwiththemembranefractionbutpp60wasnotanintegralmembraneprotein.Coronavirusescompriseagroupofen-teractionmaybearegulatorymechanismvelopedviruseswhichcauseavarie
5、tyofoperableduringvirusmaturation.diseases,usuallyconfinedtotheirnaturalStudiesontheintracellularsynthesisofhosts(2.2).ThesevirusescontainanRNAMHVproteinsbypulse-chaseexperimentsgenomeofpositivepolaritythatisap-haveshownthatthenucleocapsidprotein,proximately5.4Xlo6Dains
6、ize(5).In-pp60,isaprimarygeneproduct(3,12).IntracellularRNAreplicationisaccom-vitrotranslationindicatedthatthevirus-plishedbyavirion-inducedpolymerase(2)specificmessengerRNANo.7(1.8Xlo5whichsynthesizesafull-lengthnegativeDa)codesforaproteinwithatrypticpep-strand(8).Seve
7、ndistinctmRNAsarethentidemapidentical,orcloselyrelated,tosynthesizedfromasinglespeciesofneg-thatofvirionpp60(9,11,14,15).However,ative-strandtemplateandcompriseaproteinsoflowermolecularweightarealsonestedsetwithcommon3ends(7,8,16).synthesized,occasionallyinexcessofthePu
8、rifiedcoronavirusparticlesoftheA59virionpp60(9,12).Ithasbeensuggested(MHV-A59)straincontainthreestructuralthat
