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1、J.gen.ViroL(1978),39,545-549545PrintedinGreatBritainRibonucleoprotein-likeStructuresfromCoronavirusParticles(Accepted30Decembert977)SUMMARYThestructureoftheribonucleoprotein(RNP)complexofthreecoronaviruseswasinvestigated.Asingle-strandedhelixofdiam.I4toI6nmandupto32onminle
2、ngthwasreleasedfromdisruptedparticlesofhumancoronavirusstrain229Eandmousehepatitisvirusstrain3afterincubationinmildconditions.Thehelicalcomplexesappearedtobecomposedofglobularsubunitswithlongaxesof5to7nmsurroundingahollowcoreofdiam.3to4nm.Thecomplexeswereshowntobesensitive
3、tobothpancreaticRNaseandtopronase.Noundegradedinternalcomponentwasobtainedfromdisruptedavianinfectiousbronchitisvirusparticles.WeconcludethatthesestructuresareRNPcomplexes.ThesimilaritybetweentheseRNPsandthoseofotherlargelipidcontainingRNAvirusesisdiscussed.Coronavirusesco
4、mpriseagroupoflipidcontainingRNAvirusesthathavebeenclassifiedtogethermainlyontheiruniquemorphology(Tyrrelletak~975)-Theyappearroughlysphericalwithdiam.rangingfrom80to~2onmandcontaincharacteristicclub-shapedsurfaceprojectionsupto2onminlength(McIntosh,I974).Theenvelopeofthev
5、irusescontainslipidandappearstoconsistofadistinctpairofelectrondenseshells(McIntosh,I974).Nointernalcomponenthasbeenobservedinanynegativelystainedpreparations,althoughahelicalcomponentapparentlyreleasedfromvirionsofthehumancoronavirus,strain229E(HCV229E),hastentativelybeen
6、identifiedaspartoftheribo-nucleoprotein(RNP)complex(Kennedy&Johnson-Lussenburg,I976).ThegenomesofcoronavirusesconsistofasingleRNAmolecule.Thesehavebeenshowntobehighmol.wt.single-strandedRNAmoleculesinthecasesofHCV229E(Macnaughton&Madge,~978),HCVstrainOC43(Tannock&Hierholze
7、r,I977),avianinfectiousbronchitisvirus(IBV)(Watkinsetal.~975;Lomniczi,I977;Macnaughton&Madge,I977a;Schochetmanetal.I977)andtransmissiblegastroenteritisvirus(TGEV)(Garwesetal.I975).Furthermore,thegenomesofHCV229EandIBVhavebeenshowntocontaincovalentlyattachedpolyadenylicacid
8、sequences(Lomniczi,~977;Macnaughton&Madge,I977a,I978;Schochetmanetal.I977).Indirecteviden
