资源描述：
《冠状病毒Coronavirusl论文-2000 Replication of Enterotropic and Polytropic Murine Coronaviruses in Cultured Cell Lines of Mouse Origin_.pdf》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、Exp.Anim.49(4),251–257,2000ReplicationofEnterotropicandPolytropicMurineCoronavirusesinCulturedCellLinesofMouseOriginShigeruKYUWA1),KazutakaOHSAWA2),HiroshiSATO2),andToruURANO1)1)DivisionofMicrobiologyandGenetics,CenterforAnimalResourcesandDevelopment,KumamotoUniversity,Ho
2、njo2–2–1,Kumamoto960-0811,2)LaboratoryAnimalCenterforBiomedicalResearch,NagasakiUniversitySchoolofMedicine,Sakamoto1–12–4,Nagasaki852-8523,JapanAbstract:Tounderstandthevirus-cellinteractionsthatoccurduringmurinecoronavirusinfection,sixmurinecelllines(A3-1M,B16,CMT-93,DBT,
3、IC-21andJ774A.1)wereinoculatedwitheightmurinecoronaviruses,includingprototypestrainsofbothpolytropicandenterotropicbiotypes,andnewisolates.Allvirusstrainsproducedacytopathiceffect(CPE)withcell-to-cellfusioninB16,DBT,IC-21andJ774A.1cells.TheCPEwasinducedmostrapidlyinIC-21c
4、ellsandwasvisiblemicroscopicallyinallcelllinestested.Incontrast,thecoronavirusesproducedlittleCPEinA3-1MandCMT-93cells.Althoughmostvirus-infectedcells,exceptKQ3E-infectedA3-1M,CMT-93andJ774A.1cells,producedprogenyvirusesinthesupernatantswhenassayedbyplaqueformationonDBTce
5、lls,thekineticsofviralreplicationweredependentonboththecelllineandvirusstrain;replicationofprototypestrainswashigherthanthatofnewisolates.Therewasnosignificantdifferenceinreplicationofenterotropicandpolytropicstrains.B16cellssupportedthehighestlevelofviralreplication.Tode
6、terminethesensitivityofthecelllinestomurinecoronaviruses,the50%tissuecultureinfectiousdoseofthecoronaviruseswasdeterminedwithB16,DBT,IC-21andJ774A.1cells,andcomparedtothatwithDBTcells.TheresultsindicatethatIC-21cellswerethemostsensitivetomurinecoronaviruses.Thesedatasugge
7、stthatB16andIC-21cellsaresuitableforlarge-scalepreparationandisolationofmurinecoronaviruses,respectively.Keywords:B16cells,IC-21cells,murinecoronavirus,replication,TCID50Introductionderstood,factorssuchasthevirusstrain,doseandrouteofinfection,aswellasage,geneticbackground
8、andMurinecoronaviruses,whichareusuallycalledmouseimmunestatusofthehost,havebeenshowntobein-hepat
