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1、J.gen.Virol.(1989),70,1713-1724.PrintedinGreatBritain1713Keywords:coronavirus/ratcelllines~internalizationSeveralRatCellLinesShareaCommonDefectinTheirInabilitytoInternalizeMurineCoronavirusesEfficientlyByWAYNEF.FLINTOFF*ANDSHEILAVANDINTERDepartmentofMicrobiologyandImmunology,HealthS
2、ciencesCentre,UniversityofWesternOntario,London,OntarioN6A5C1,Canada(Accepted3March1989)SUMMARYInfectionofratcells,SchwannomaRN2,hepatomaHTCormyoblastL6,withthemurinecoronavirusJHMstrainresultsinapersistentinfectioncharacterizedbythereleaseofvirusoveranextendedperiodoftimewithalimit
3、edcytopathology.SeveralstagesoftheviralreplicationcyclehavebeenexaminedinthesecellsincomparisontothoseinmouseL2cells,whicharetotallypermissivetoJHMinfection.Althoughtheratcellsboundasmuchvirusasthemousecellstheirabilitytointernalizeitwas40-foldlessefficientthanthemousecells.Thislowe
4、rinternalizationefficiencywasnotenhancedbypHshockofinfectedcells,butwasbytreatmentwithpolyethyleneglycol.InallcelltypesthereappearedtobenomajordifferencesintheabilityoftheinternalizedvirustoreplicatetheviralRNAasdeterminedbyslot-blotanalysiswitharadiolabelledviralcDNA.Asimilargeneti
5、cmechanismappearstobeoperativeinthelinesbecausesomaticcellhybridsformedbetweentheselinesinvariouscombinationswerealsodeficientintheabilitytointernalizeboundvirus.Takentogethertheseresultsimplythatratcelllinesingeneralshareacommondeficiencyintheirinabilitytointernalizemurinecoronavir
6、usesefficiently.Thislowefficiencyinviralinternalizationmayexplaininparttheabilityoftheselinestosustainpersistentinfections.INTRODUCTIONInrecentyearsmembersofthemurinecoronaviruseshavebeenstudiedextensivelybecauseoftheirabilitytoestablishpersistentinfectionsbothinvitro(Holmes&Behnke,
7、1981;Stohlman&Weiner,1978;Lucasetal.,1977,1978;Leibowitzetal.,1984;Lamontagne&Dupuy,1984)andinvivo(Knobleretal.,1981;Hiranoetal.,1980;Nagashimaetal.,1979;Sorensenetal.,1980,1982,1984).Becauseofthisproperty,theseagentshavebeenconsideredasusefulmodelsforseveralchronicdiseasestatesincl
8、udingdemyelinatingd
