冠状病毒Coronavirusl论文-1991 A Golgi Retention Signal in a Membrane-Spanning Domain of Coronavirus E1 Protein.pdf

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1、PublishedOctober1,1991AGolgiRetentionSignalinaMembrane-spanningDomainofCoronavirusElProteinAnnM.SwiftandCarolynE.MachamerDepartmentofCellBiologyandAnatomy,TheJohnsHopkinsUniversitySchoolofMedicine,Baltimore,Maryland21205Abstract.TheElglycoproteinfromanaviantheirsinglemembrane-spanningdomains

2、werere-coronavirusisamodelproteinforstudyingretentionplacedwiththefirstmembrane-spanningdomainfromintheGolgicomplex.InanimalcellsexpressingtheEl.SingleaminoacidsubstitutionsinthissequenceproteinfromcDNA,theElproteinistargetedtocisreleasedretentionofthechimericGprotein,aswellGolgicisternae(Ma

3、chamer,C.E.,S.A.Mentone,asamutantElproteinwhichlacksthesecondandJ.K.Rose,andM.G.Farquhar.1990.Proc.Natl.thirdmembrane-spanningdomains.Theimportantfea-Acad.Sci.USA.87:6944-6948).Weshowthatthetureoftheretentionsequenceappearstobetheun-Downloadedfromfirstofthethreemembrane-spanningdomainsofthec

4、hargedpolarresidueswhichlineonefaceofapre-Elproteincanretaintwodifferentplasmamembranedictedalphahelix.ThisisthefirstretentionsignaltoproteinsintheGolgiregionoftransfectedcells.BothbedefinedforaresidentGolgiprotein.ThefactthatitthevesicularstomatitisvirusGproteinandthealpha-ispresentinamembr

5、ane-spanningdomainsuggestsasubunitofhumanchorionicgonadotropin(anchoredtonovelmechanismofretentioninwhichthemembranethemembranebyfusionwiththeGproteinmem-compositionoftheGolgicomplexplaysaninstrumen-brane-spanningdomainandcytoplasmictail)werere-talroleinretainingitsresidentproteins.jcb.rupre

6、ss.orgtainedintheGolgiregionoftransfectedcellswhenORTINGofnewlysynthesizedproteinsintheexocyticTheGolgicomplexplaysacentralroleintheprocessingpathwayisafundamentalproblemincellbiologywhichandsortingofnewlysynthesizedproteins(reviewedinrefer-onApril3,2015Shasreceivedagreatdealofattentioninrec

7、entyears.ence9).Itscharacteristicmorphology(stacksofflattenedSecretedandplasmamembraneproteinsfollowacommonsaccularmembranes)andcentrallocation(peri-orjuxtanu-pathwaythroughthecell:fromtheER,throughtheGolgiclear)inthecellmaybeimportantforthesefunct