冠状病毒Coronavirusl论文-2004 Primary Characterization of SARS Coronavirus Strain Frankfurt 1.pdf

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1、DokladyBiologicalSciences,Vol.394,2004,pp.58–60.TranslatedfromDokladyAkademiiNauk,Vol.394,No.4,2004,pp.566–568.OriginalRussianTextCopyright©2004byAgafonov,Gus’kov,Ternovoi,Ryabchikova,Durymanov,Vinogradov,Maksimov,Ignat’ev,Nechaeva,Netesov.GENERALBIOLOGYPrimaryCharacterizationofSARSCoronavirusStrai

2、nFrankfurt1A.P.Agafonov,A.A.Gus’kov,V.A.Ternovoi,E.I.Ryabchikova,A.G.Durymanov,I.V.Vinogradov,N.L.Maksimov,G.M.Ignat’ev,E.A.Nechaeva,andCorrespondingMemberofRASS.V.NetesovReceivedAugust7,2003Severeacuterespiratorysyndrome(SARS)oratyp-thedisruptionofthemonolayerbecauseoflocalcellicalpneumoniawasfirst

3、diagnosedinGuangdonglysis.VirusreproductionincellswasmonitoredbytheProvince(China)inNovember2002.AccordingtoRT–PCRmethod.AmplificationwasconductedasWHOdata,patientswithatypicalpneumoniaappeareddescribedpreviously[7]usingtheBNIinSandBNIi-in29countriesthroughouttheworldbymid-2003.BynAsprimersdeveloped

4、intheInstituteofTropicalMed-June2003,thetotalnumberofaffectedpersonsreachedicine(Hamburg,Germany)[7].Thesamplestakenfrom8435,including789lethalcases[1].TheSARScaus-allpassagesofthecellsstudiedwerefoundtocontainativeagentwasfoundtobeavirusfromgenusCoro-theSARS-virusRNA.navirusoftheCoronaviridaefamil

5、y[2].MembersofElectron-microscopicexaminationrevealedcoro-genusCoronavirushavebeenknownforalongtime.navirusreproductioninculturesoftheVeroandTheseareenveloped,positive-strandedRNAviruses293linesonthesecondandthirddaysafterinoculation,thatcausehumanandanimaldiseases[3,4].However,respectively.Theendo

6、plasmicreticulumoftheinfectedsuchaseverecoronavirusinfectionhavebeenprevi-cellscontainedparticleswithamorphologytypicalofouslyextremelyrareinhumans.Becauseofthehighcoronavirus:longsurfaceprojections,acoilednucle-riskofatypicalpneumoniaepidemic,thepropertiesofoid,andanelectron-transparentcore(Fig.1a

7、).VirionSARSvirus,anewmemberofthisgenus,arebeingaccumulationwasalsoobservedintheperinuclearstudiedextensively[5–8].spaceandsmooth-membranevacuoles.IncellsoftheOurstudywasthefirsttoanalyzethebasicproper-Veroc