冠状病毒Coronavirusl论文-2009 Structure of the X (ADRP) domain of nsp3 from feline coronavirus.pdf

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1、researchpapersActaCrystallographicaSectionDStructureoftheX(ADRP)domainofnsp3fromBiologicalCrystallographyfelinecoronavirusISSN0907-4449JustynaA.Wojdyla,aIoannisThestructureoftheX(orADRP)domainofapathogenicReceived24June2009abvariantoffelinecoronavirus

2、(FCoV)hasbeendeterminedinAccepted1October2009Manolaridis,EricJ.Snijder,AlexanderE.Gorbalenya,bBrunotetragonalandcubiccrystalformsto3.1and2.2A˚resolution,Coutard,cYvonnePiotrowski,drespectively.Inthetetragonalcrystalform,glycerol-3-PDBReferences:FCoVXR

3、olfHilgenfelddandPaulA.phosphatewasobservedintheADP-ribose-bindingsite.Bothdomain,3ew5,r3ew5sf;3eti,acrystalformscontainedlargesolventchannelsandhadar3etisf;ADP-ribose-bound,Tucker*solventcontentofhigherthan70%.Onlyveryweakbinding3jzt,r3jztsf.ofthisdo

4、maintoADP-ribosewasdetectedinvitro.However,aEMBLHamburgOutstation,c/oDESY,thestructurewithADP-riboseboundwasdeterminedintheNotkestrasse85,D-22603Hamburg,Germany,cubiccrystalformat3.9A˚resolution.ThestructureofthebMolecularVirologyLaboratory,Department

5、ofFCoVXdomainhadtheexpectedmacro-domainfoldandisMedicalMicrobiology,CenterofInfectiousthefirststructureofthisdomainfromacoronavirusbelongingDiseases,LeidenUniversityMedicalCenter,POBox9600,2300RCLeiden,tosubgroup1a.cTheNetherlands,LaboratoireArchitectu

6、reetFonctiondesMacromole´culesBiologiques,UMR6098,AFMB–CNRS–ESIL,Case925,163AvenuedeLuminy,13288Marseille,dFrance,andInstituteofBiochemistry,CenterforStructuralandCellBiologyinMedicine,UniversityofLu¨beck,RatzeburgerAllee160,23538Lu¨beck,Germany1.Intr

7、oductionCoronavirusesarepositive-strandedRNAvirusesthatbelongtotheorderNidovirales(Gorbalenyaetal.,2006).TheirvirionCorrespondencee-mail:tucker@embl-hamburg.derangesfrom80to120nmindiameterandcontainsonemoleculeofRNAofapproximately30kb(Weiss&Navas-Mart

8、in,2005).TranscriptionandreplicationofthecoronaviralRNAgenometakesplaceinthecytoplasmofthehostcellatreplicationsitesthatareassociatedwithacharacteristicreti-culovesicularnetworkofmodifiedendoplasmicreticulum(Stertzetal.,2007;Snijderetal.,2006;P