地塞米松降低肺癌细胞对顺铂化疗敏感性的机制与分析-论文.pdf

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1、·942·现代中西医结合杂志ModernJournalofIntegratedTraditionalChineseandWesternMedicine2014Mar，23(9)地塞米松降低肺癌细胞对顺铂化疗敏感性的机制与分析周琴，郭光云，尹义平(湖北医药学院附属人民医院，湖北十堰442000)[摘要]目的探讨地塞米松对顺铂治疗肺癌时诱导的细胞凋亡的影响及其机制。方法将人肺癌细胞A549分为单纯顺铂组、顺铂联合不同浓度地塞米松处理组(联合组)，分别检测处理后细胞的增殖情况。采用RT—PCR检测DNA损伤信号通路及细胞N

2、F—KB信号通路上的相关基因p53和cyclinD1的表达情况。结果与单纯顺铂组相比，联合组提高了肿瘤细胞的存活率，抑制了由顺铂诱导的细胞凋亡现象。联合处理后的肿瘤细胞中关键因子抑癌基因p53表达上调和肿瘤细胞中原癌基因cyclinD1的表达下调的效应均受到抑制，从而表现出肿瘤细胞对化疗药物顺铂失敏的现象。结论地塞米松通过干扰化疗药物对肿瘤细胞的抑制作用而降低了化疗药物顺铂对肺癌细胞的敏感性，这其中部分原因是由于下调了p53所致。提示临床肿瘤治疗方案中，糖皮质激素与抗癌药物的联合使用应"3-慎重。[关键词]肺癌；化疗

3、；顺铂；糖皮质激素doi：10．3969／j．issn．1008—8849．2014．09．010[中图分类号]R734．2[文献标识码]A[文章编号]1008—8849(2014)09—0942—03Mechanismandanalysisofdexamethasoneinreducingcis-DichlorodiammineplatinumchemotherapysensitivityoflungcancercellsZhouQin，GuoGuangyun，YinYiping(ShiyanPeople’SHos

4、pitalofHubeiMedicalUniversity，Shiyan442000，Hubei，China)Abstract：ObjectiveItistoapproachtheinfluenceandmechanismofdexamethasone(DEX)Oflungcancercellssensi—tivitytocis—Dichl0r0diammineplatinum(DDP)．MethodsHumanlungcancercellA549wasdividedintoDDPgroupandDDPcombine

5、dwithdifferenceconcentrationDEXgroups(combinedgroup)，andtreatedwithDDPinthepresenceorabsenceofdifferentconcentrationofDEX．ThenumberofviablecellandthecellproliferationwasmeasuredbytheMT—assay；Ex—pressionofDNAdamagesignalingandcellularNF—KBsignalingpathwayrelated

6、genesp53andcyclinD1weredetectedbyRT—PCR．ResultsComparedwithDDPgroup，thesurvivalrateoftumorcellsincombinedgroupwasimproved，andtheap—optosisreducedbycisplatinwasinhibited．Aftercombinedtreatmentoftumorceils，theexpressionofkeyfactorgeneofDNAdamagesignalingpathway—p

7、53wassuppressed．andtheexpressionofcyclinD1一thekeygeneofNF—kBsignalingpathwaywasupregulated．ConlYlusionDEXcaninterferesthedepressanteffectofchemotherapydrugsontumorcellsandreducethesensitivityoflungcancercellsforchemotherapy，whichisdueinparttocutp53induced．Sothe

8、combineduseofglucocorti—coidandanticancerdrugsshouldbecarefulinclinicaltumortreatment．Keywords：lungcancer；chemotherapy；cis—Dichlorodiammineplatjnum；dexamethasone糖皮质激素如地塞米松(D