吡格列酮对非酒精性脂肪肝病大鼠Kupffer细胞的影响-论文.pdf

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1、·886·2014年7月第16卷第7期JournalofPhYBic塑Ylv：，：!·论著·吡格列酮对非酒精性脂肪肝病大鼠Kupffer细胞的影响贺丹李岚刘慧霞【摘要】目的探讨吡格列酮对sD大鼠非酒精性脂肪肝病形成的预防作用及其机制。方法36只雄性sD大鼠按随机数字表法分为正常对照组、高脂饮食组及吡格列酮干预组，饲养8周；正常对照组喂饲普通饲料，剩余两组喂饲高脂饲料；吡格列酮干预组于实验第4—8周予吡格列酮灌胃，其余两组同期予蒸馏水灌胃；测定空腹血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、甘油三酯(TG)、总胆固醇(Tc)、空腹血糖(FPG)、空腹胰岛素(FIN

2、S)水平；计算空腹胰岛素抵抗指数(FIRI)；HE染色分析肝组织切片病理学改变；观察Kupffer细胞形态变化及分泌肿瘤坏死因子一0【(TNF-d)、一氧化氮(NO)的水平。结果高脂饮食组大鼠空腹FIRI、TG、TC均高于正常对照组，差异均有统计学意义(P

3、本正常。结论吡格列酮可预防高脂饮食诱导的非酒精性脂肪肝病发生；其机制可能与改善胰岛素抵抗、降低血脂和调节Kupffer细胞功能有关。[关键词]噻唑烷二酮类／药理学；脂肪肝；枯否细胞；胰岛素抗药性；大鼠，Sprague-DawleyTheefectsofpiogUtazoneontheKupfercellsofnonalcoholicfattyliverdiseaseinratsHeDan，LiLan，LiuHuixia,．DepartmentofEndocrinology，CentralHospitalofXiangtanCity，Xiangtan411100，ChftaCorre

4、spondingauthor：LiuHuixia，Email：lhx900@yahoo．corn．cn【Abstract】ObjectiveToexplorethepreventiveeffectandmechanismofpioglitazoneonnonalcoholicfattyliverdiseaseinrats．MethodsThirtysixmaleSprague-Dawley(SD)ratswererandomlydividedintonormalcontrolgroup，high-fatdietgroup，andpioglitazoneinterventiongro

5、up，with12ratsineachgroup．Normalcontrolgroupfedwithstandarddietforeightweeks，andtheresttwogroupsfedwithhighfatdiet．Pioglitazoneinterventiongroupfedwithpioglitazoneinthefourtoeightweeks，theresttwogroupsinthesameperiodfedwithdistilledwater．Fastingserumalanineaminotransferase，aspartateaminotransfe

6、rase，totalcholesterol，triglye-erides，fastinguc0se，andfastingbloodinsulinlevelsweredetermined．Thefastinginsulinresistanceindexwascalculated．Routinehistologicalfeaturesofhepaticsectionwereobservedbyhematoxylineosin(HE)staining．TheshapechangeofKupffercellsintheliv-erwasdetected．TIhelevmls0ftumorn

7、ecrosisfactor(TNF)nandnitricoxide(NO)secretedbyKupffercellsweremeasured．ResultsThefastinginsulinresistanceindex，triglyeerides，andtotalcholesterolinratsofhigh—fatdietgroupweresignificantlyhigherthaninnormalcontrolgroup(P<0．05)．Hi每topath0