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页数:8页
时间:2020-05-18
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1、step1:Thisisadorsalviewofamouse’sskull,inordertoaccessthebrainyoucan cutalongthecoronalsutureandsagittalsuturethenpulloffbothsidesofparietal boneandinterparietalbonestep2:Nowtheskullisopenandyoucanseethebrainclearly,inordertogetthe hippocampusyou’dbetterkeepthebraininthecranialcavi
2、tysothebrainwillnotmovewhenyoucontinuewiththefollowingsteps 0step3:Inordertoexposethehippocampusyouneedtoremovethecerebralcortexcoveringit.Thefirstincisionisattheendofthehemisphere;theincisionshouldbe about0.7mmdeepformostadultmousethatyoumightnothurtthehippocampus whiletoexposeit.
3、The2ndincisionisabout1.5-2mminfrontofthefirstone,this incisionyouneedcutintothelateralventricle,bothoftheincisionsgototheventralofthebrainandmeetthere.Nowthispieceofcortexisfree,pullitup,youwillsee thehippocampusjustlikeinthispicture,alsoyoucanseetheCSFintheopened ventricle. 0step4
4、:Keepworkingontheothersideofthebrainpullupbothsidesofthecortexthatcoveringthehippocampusalongtheventricle.Nowyoucanseethedorsalpart ofthehippocampus.Separatetherestofthehippocampusfromthecortexcovering italongthesurfaceofthehippocampustowardstheventralpartofthehippocampus. 01票step5
5、:Keepworkingontheothersideofthebrainpullupbothsidesofthecortexthatcoveringthehippocampusalongtheventricle.Nowyoucanseethedorsalpart ofthehippocampus.Separatetherestofthehippocampusfromthecortexcovering italongthesurfaceofthehippocampustowardstheventralpartofthehippocampus. 0step6:N
6、owyouneedtofreethehippocampusfromthesurroundingtissue 0step7:Hereisthehippocampuspickedoutfromthebrain. 0Thehippocampalformationisabilateralstructuresandwichedbetweenthecerebralcortexandthethalamus.Figure1depictsareconstructionofthehippocampus(yellow)initsthree-dimensional(3D)posit
7、ionwithintherat'sbrain.These3DreconstructionsweremadefromtheserialsectionsofLONI'sRatBrainAtlas. 0
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