川牛膝多糖对衰老小鼠模型的体内抗氧化作用.pdf

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1、中国抗生素杂志2014年7月第39卷第7期553文章编号：1001—8689(2014)07．0553—07川牛膝多糖对衰老小鼠模型的体内抗氧化作用李俊丽韩兴发刘铁秋曾宪垠(四川农业大学生命科学院原子能农业应用研究室，雅安625014)摘要：目的探讨川产道地中药材川牛膝多糖(RCPs)对衰老小鼠模型的体内抗氧化作用。方法采用D．半乳糖注射诱导衰老模型，同时给予RCPs或维生素C(Vc)，连续处理42日后检测小鼠肝脏、心脏、脑组织抗氧化指标。结果与模型组相比，灌胃RCPs或Vc均显著增加小鼠脾脏和肝脏指数(尸<0．05

2、)；增强肝、心、脑总抗氧化能力(T-AOC)、超氧阴离子及羟自由基清除能力(尸l

3、作为一种优良的天然抗氧化剂来源。关键词：川牛膝；多糖；自由基；抗氧化活性；mRNA中图分类号：Q949．95文献标志码：AEffectsofp0lysaccharidesfromtherootsofRadixCyathulaeoficinalisKuanon／nvivoantioxidantcapacitiesofD-galactose-inducedagingmousemodelLiJun—li，HanXing—fa，LiuTie-qiuandZengXian—yin(IsotopeResearchLaborata

4、ry,Collegeoflifescience，SichuanAgricuturalUniversity,Ya’an625014)Abstract0bjectiveToinvestigatetheeffectsofpolysaccharidesfromtherootsofSichuanlocalRadixCyathulaeOficinalisKuan(RCPs)oninvivoantioxidantcapacitiesofmiceagedbyD—galactose．MethodsMiceweregivendailys

5、ubcutaneousinjectionofD．galactose，toinduceanagingmousemodel，andtheircounterpartsweredailyiniectedwithD—galactoseandsimultaneouslyorallyadministratedwitlleitherRCPsorvitaminC(Vc)．Alldrugswereadministratedfor42days。thenantioxidantparametersinmice1iver,heartandbra

6、intissuesweretested．ResuitsComparedwiththemode1group．ora1administrationofD—galactose—inducedagingmicewitheitherRCPsorvitaminCfVc1bothsignificantlyincreasedspleenandliverindices(P<0．05)，andthetotalantioxidantcapacities(T-AOC)，superoxideanionandhydroxylradica1sca

7、vengingcapacitiesofliver,heartandbraintissues(P<0．05)；enhancedtheantioxidantenzymeactivitiesincludingtotalsuperoxidedismutase(T-SOD)，glutathioneperoxidase(GSH—Px)，andcatalase(CAT)inliver,GSH—PxandCATinheart，T-SODandGSH．Pxinbrain(JP<0．O5)；decreasedmalondialdehyd

8、efMDA)concentrationsinliver,heartandbrain(P<0．05)．Furthermore，mRNAexpressionlevelsofCu，Zn-SOD，Mn—SOD．GSH—Pxinliver,heartandbrain．andCATinliverandheartweresignificantlyhigher