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1、第24章GenediagnosisSectiononeWhatisgene?1.Historyofgeneresearch(1)In1866,Mendelsuggestedanewconceptionofgenetics,orgeneticfactorbypeahybridizationtest.(2)In1889,Johonsensuggestedaword,gene,tostandforgeneticfactor.(3)In1926,Morgensuggestedgenetheory.(Hewroteinhisbook:Genetheoryhads
2、hownthatgenesweresomegeneticpelletsonchromatin.)4.In1944,AveryprovedthatDNAisgeneticsubstancebybacteriatransformationtest.5.In1946,Lederbergfoundgenerecombinationphenomenon.6.In1953,WatsonandCricksuggestedDNAdoublehelixstructure2.Whatisgene?Geneisafunctionalfragmentofnucleicacid
3、.SectiontwoWhatisgenediagnosis?1.Howmanykindsofnucleicacidarethere?2.Whatisdifferencebetweengeneandnucleicacid?Nucleicacidlikesalongriver.Whileagenelikesasprayinlongriver.3.WhydoesgeneincludeRNA?Thereasonsare:(1)RNAinRNAviruscaninverse-transcribedintoDNA,theycanalsotranslatedint
4、oprotein.(2)Hetero-nucleicRNA(hnRNA)andmRNAplaygene’sfunction.4.Whatissamepointbetweennucleicacidandgene?Theyarenucleicacid,butDNAislong,geneisshort.5.Whatisgenediagnosis?(Genediagnosisisakindofanalysistogene,ornucleicacidfragment,whichinducedisease)Section3Whatissignificanceofg
5、enediagnosis?1.Diagnosistovariousdisease2.Usageonmedicaljurisprudence(1)Identificationofbaby(2)Identificationofvarioussamplesfromthesceneofcrime,suchasblood,bone,muscleandhair,etc.(Whoismurderer?)3.Geneidentificationofvariousplants,andherbsformedicineSection4Howisgenediagnosisdo
6、ne?1.SometechnologiesforDNAanalysis(1)Restrictionfragmentlengthpoly-morphorism(RFLP)analysis------------//---------//--------------------//-------------------(2)Singlestrandconformationpoly-morphorism(SSCP)analysis----------------------------------------------(3)DNASequencinga.S
7、omemainmethodsforDNAsequencing(a)Maxium’schemicalmethod(b)Sanger’sdi-deoxygenmethod(c)DNAsequencinganalyzer组合酸性肽基因克隆的测序结果P1NTNTCTGACTTAACCTATAAAAATANNCGTATCACGAGGCCCTTTCGTCTTCACCTCGAGAAATCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATAGATTCAATTGTGAGCGGATAACAATTTCACACAGAATTCATTAAAGAG
8、GAGAAATTAACTATGAGAGGATCGCATCACCATCACCATCACGGATC
