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1、棉花学报CottonScience2013袁25渊4冤院300~308利用高通量测序技术鉴定棉纤维发育相关miRNAs及其靶基因1,222222221,2*南文智袁吴嫚袁于霁雯袁范术丽袁黄双领袁李兴丽袁张红卫袁张金发袁喻树迅渊1.西北农林科技大学农学院袁陕西杨凌712100曰2.中国农业科学院棉花研究所/棉花生物学国家重点实验室袁河南安阳455000冤摘要院miRNA渊microRNA冤是一类21～24个核酸长度的非编码小分子RNA渊sRNA冤袁它主要通过抑制或降解靶基因来调控植物生长发育等过程遥试验利用在纤维长度上有显著差异
2、的2个回交自交系BILs渊Backcrossinbredlines冤的0DPA渊Dayspostanthesis冤尧3DPA的胚珠和10DPA的纤维构建6个sRNA文库并进行Solexa测序遥以已公布的棉花D5基因组序列和棉属其他序列为参考袁经分析共发现561个miRNAs袁其中包括254个已知的miRNAs渊属于40个miRNA家族冤袁75个候选的miRNAs和232个新的miRNAs袁研究结果极大地丰富了棉属miRNAs遥通过miRNA靶基因预测分析发现多数miRNAs负调控其对应的靶基因袁少数正调控遥KEGG渊Kyoto
3、encyclopediaofgenesandgenomes冤注释结果表明miRNA的靶基因在植物激素代谢途径中显著富集遥关键词院棉花miRNA曰高通量测序曰棉花D5基因组曰靶基因预测中图分类号院S562.035.3文献标志码院A文章编号院1002-7807(2013)04-0300-091,222222NANWen-zhi,WUMan,YUJi-wen,FANShu-li,HUANGShuang-ling,LIXing-li,ZHANG221,2*Hong-wei,ZHANGJin-fa,YUShu-xun(1.712100,
4、;2.455000)miRNAs(microRNAs)are21-24ntnon-protein-codingsmallRNAsthatplayimportantrolesincontrollingplantgrowthanddevelopmentbydirectingtargetmRNAcleavageortranslationalinhibition.Inthisstudy,weconstructedlibrariesus-ingovulescollectedat0DPA(dayspostanthesis)and3DPA,
5、andfiberscollectedat10DPA,fromtwointerspecificbackcrossinbredlines(BILs).Thelibrariesweresequencedandanalyzed.Thereweresignificantdifferencesinfiberlengthbetweenthetwolines,whileotheryieldandfiberqualitycharactersweresimilar.BycomparisonwiththeD5cottongenomeandother
6、knowncottonsequences,weidentified561miRNAs,including254conservedmiRNAs,75candidateconservedmiRNAs,and232newmiRNAsinthelibraries.ThesedatarepresentalargeincreaseinthenumberofknowncottonmiRNAs.MostmiRNAsactasnegativeregulatorsoftheirtargets,andveryfewmiRNAsarepositive
7、regulators.AnalysesoftargetgenefunctionsindicatedthatplanthormonemetabolismwasthemaintargetformiRNAs.KEGG(Kyotoencyclopediaofgenesandgenomes)analysisindi-catesthatmiRNAtargetgenesweresignificantenrichedinplanthormonepathways.cottonmiRNAs;deepsequencing;cottonD5genom
8、e;targetprediction棉花是世界最重要的棉纺织工业原料袁具有miRNAs渊microRNAs冤在近年来受到研究人员的重要的经济价值遥棉纤维是由胚珠表皮细胞经分广泛关注遥miRNA是一类内源非编码小RNA化起始尧伸长尧次生壁增厚和脱水成熟形成的单渊ncRNA冤袁主
