兰科植物常用培养基配制成分

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1、成分分子量使用浓度(mg/L)大S元素硝酸钾KN03101.111900硝酸铵NH4N0380.041650磷酸二氢钾KH2P04136.09170硫酸镁MgS04.7H20246.47370氯化钙CaC12.2II20147.02440微量元素碘化钾KI166.010.83硼酸H3BO361.836.2硫酸锰MnS04.4H20223.0122.3硫酸锌ZnS04.7H20287.548.6钼酸钠Na2Mo04.2H20241.950.25硫酸铜CuSOd.5H20249.680.025鉍化钴CoC12.62237.930.025铁盐乙二胺四乙酸二钠Na2.RDTA372.2537.3硫酸

2、亚铁FeSO24.7H20278.0327.8有机成分肌醇100甘氨酸2盐酸硫胺素VB10.1盐酸毗哆醇VB60.5烟酸VB5或VPP0.5庶糖sucrose342.3130g/L琼脂agar7g/L成分分子量使用浓度（mg/IJ大量元素硫酸铵（NH4）2SO4132.15500磷酸二氢钾KH2PO4136.09250硫酸镁MgS04.7H20246.47250硝酸钙Ca3(P04)2236.161000微量元素硫酸锰MnSOd.4H20223.017.5硫酸亚铁FeSO24.7H20278.0325鹿糖sucrose342.3120g/L琼脂agar15g/L注：大量用于兰花各属无菌种子发

3、芽和组织培养，琼脂在使用时可酌减。摘自：《观赏植物组织培养技术》ND培养基配方成分分子量使用浓度（mg/IJ大量元素硝酸钾KNO3101.11200硝酸铵NH4NO380.04480硝酸钙Ca(N03)2.4H20236.16470氯化钾KC174.55150磷酸二氢钾KII2P04136.09550硫酸镁MgS04.7H20246.47250微M元素硼酸H3B0361.830.5硫酸锰MnS04.4H20223.013.0硫酸锌ZnSOd.71120287.540.5钼酸钠Na2Mo04.2H20241.950.025硫酸铜CuS04.5H20249.680.025氯化钴CoC12.622

4、37.930.025浓硫酸ConcentratedH2S040.5ul铁盐乙二胺四乙酸二钠Na2.EDTA372.2537.3硫酸亚铁EcS024.7H20278.0327.8有机成分肌醇Myo-inositol100盐酸硫胺素ThiaminIIC1VB11.0盐酸毗哆醇PyridoxinHC1VB6205.61.0烟酸NicotinicacidVB5或VPP123.111.0泛酸韩Calciumpantothenate1.0腺嘌呤Adenine1.0L-半胱氨酸L-cysteine1.0生物素D-biotin0.1庶糖sucrose342.31g/L琼脂agarg/L注：蝴蝶兰组培用摘白：

5、MicropropagationofPhaleanopsisandDoritaenopsisbyculturingshoottipsofflowerstalkbuds.Plantcellreport(1993)13:7-11VW培养基配方成分分子董使用浓度(mg/L)大量元素硝酸钾KNO3101.11525硫酸铵(NH4)2S04132.15500磷酸二氢钟KH2P04136.09250硫酸镁MgS04.7H20246.47250过磷酸钙Ca3(P04)2200微量元素硫酸锰MnS04.4II20223.017.5草酸铁Fe2(C4H406)328庶糖sucrose342.3120g/L琼脂

6、agar18g/L通用铁盐乙二胺四乙酸二钠Na2.EDTA372.2537.3硫酸亚铁FeS024.7H20278.0327.8注：1、现在一般用通用铁盐代替草酸铁。2、过磷酸钙先用盐酸溶解，再加入培养基屮。3、现在琼脂质量较好，具体用量，自己掌握。4、用于兰花播种和组织培养，具有较好的缓冲能力。摘自：somgphchangesinnutrientsolutions.Note:MediacontainingPhytagelasthegellingagentmustbepouredjustafterautoclaving.Thismediumcannotbere-meltedafteritha

7、ssolidified.Allthemediaarepreparedfromstocksolutionsofmacroelements，microelementsandvitamins.Thesestocksolutionsarestoredat-20°Cin50mlaliquots-Thegrowthregulators,trans-zeatinandIAB,andtheantibiotics,kanamy