爱沙木组织培养与植株再生研究

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1、摘要本文以Eremophitabignoni毋Ol't2penth，JFMuell，的穗子、Eremophilamacdon—nelliiFMuetL的予叶和光秃爱沙木Eremophilaglabra(stickygreenleaf)的扦插藩豢芽茎段为舶棱俸送行缝织墙葵擅接孬生硬究。羞重磺究了壤拣霉生过程中的确动培养、有效芽诱导、生根培养等各个阶段的影响和制约因素，主幕包括基本壤纂鏊、生长调节裁、糖、溪涯炭等，礁囊壅它弱在各令绪养狳羧酶最佳培莽基配方。并以Eremophilabignoniiflor

2、a(benth．●FMuell无菌蒴带芽鍪段增熊培养居获譬豹生蔽蕊进J嚣豫蔼。主要磺究结票翅下：MS培辨基是种子和子叶启动培养中的效果都较好的基本培养基，种子萌发和予时分纯最适寤动培养蕊相阊均为MS*BAl．0mg／L+NAA0．Img／L。而B5培养基在扦插替带芽茎段的启动培养中效果较好，最邋启动壤养基为B；+BA0．5mg／L+NAA0．Img／L。种子、子叶和扦插稿带芽蒸段的启动率分别达到72．70％、7170％和79．70％。Eremophilabignoniiflora(benth．jFM

3、uelL无菌苗带芽蒸段有效芽诱导的最佳培棼基配方为MS+BAl。0mg／L+NAA0．1mg／L。谚蝰葬基上奏效劳诱导率达73，0s％。60d时统计，增殖倍数达5．5；EremophilamacdonnelliiFMuell子叶诱导有效芽熬最毽缝合戈麦芽糖+BAt．0mg／L+NAA0。lmg／L，该蹬养基上有效努诱导攀达7227％。60d时统计，增殖倍数达5．0；光秃爱沙木扦插苗带辩茎段有效芽诱导豹最佳潦养莲醚方为KT0。5mg／L+BA0．5mgtL+IBA0。05mg／L。该培养鏊上的诱导举达

4、67．23％。60d时统计，增魑倍数达4．3。Eremophilabignoni(露口r4penth．jEMuelL生檄培养酌最饶配方为：1／2MS+NAAO．5mg／L+IBAO．05mg／L+1％活性炭，21d时统计生根率为81．6％，缎培茁完整植株的平均裉数为3．9，平均粮长为3．1cm。Eremophilabignoniiflora(benth,)FMuelL最毽炼萤基质为珍球装：蛭石：溺沙=3：2：1，成活率达到80％以上。奁试验过程中，发瑗了予叶意接再生劳这孝孛本本檀物舔毒黪檀撩嚣生方式

5、。关键逶：鬣沙本缝缀壤莽{耋拣菇生StudiesonTissueCultureandPlantletRegenerationofEremophilaspp．ZhangJiankang(ForestryCulture)DerectedbyProf．ShiDaxin91＆AssociateProf．LuoJianxun2(1．CollegeofForestryandHorticulture，SichuanAgriculturalUniversity，Ya’an，Siehuan，China，6250142

6、．TheResearchlnstuteofForestry，SichuanAcademyofForestry，Chengdu，Siehnan，China，610081)AbstractThetissuecultureandplantletregenerationfromseedofEremophilabignoni够·ora阳enth．JFMuelI．，cotyledonofEremophilamacdonnelliiEMuellandsproutexplantswhichwerefromcutti

7、ngplantofEremophitaglabrafR3t)Ostenf(stickygreenLeaf)werestudied．Theinfluencefactorsofplantletregenerationwerestudiedduringtheinitiaiculture，budinductionandrootingculture。Throughanalyzingthebasalmedittm，phyto—hormone，saccharideandactivatedcharcoal，theo

8、ptimummediu—mwasconducted．Thetransplantationtrialofthetest—tubeplantletwhichderivedfromthebacteria—freeseedlingofEremophilabignoni孵orapenth．JEMuellwasmadeatlast．Themainresultsofthispaperwereconductedasfollows：MSW'aSthebestbasalmediumins