COX2在裸鼠卵巢癌移植瘤中的表达及其抑制剂对卵巢癌生长的抑制作用

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1、COX·2存裸鼠卵巢痛移植瘸中的表迭腱艇抑制剂神：佛内外对卵巢癌生长的抑制作用摘鞭中文摘要目的研究裸鼠卵巢移植瘸缀织中COX．2的表达，及其抑制剂对人卵巢癌细胞株H08910生长的影响以及对人卵巢癌裸鼠移植瘤生长的抑制效应。方法采弱MTT法磷究选耩经COX．2移鞠裁薅H08910生长数彝乡}静剿偿爰。鼷察COX．2抑制剂对裸鼠卵巢原位移植瘤生长的体内抑制作用。免疫缀织化学染色检测移植瘤细胞增殖核抗原(PCNA)及环氧合酶．2(COX一2)的表达。逆转录聚合酶毯茨应(RT．PCR)疆究禳簸黪巢琢位移攘瘘COX-2熬豢运及COX-2麴翻翔对羚鬃原位移植瘤C

2、OX．2表达的影响。结粜(1)免疫组织化学染色结果表明肿癌组织韵PCNA和COX-2阳性表达3锎，强鼯萑7铡，PCNA鞠瞧袭这2铡，瀵羯毪8溺。怒灌组COX-2裘这下调(P<0．05)。(2)RT—PCR结果表明处理缎的COX-2表达明显下调(P

3、=i坚塑墅墅型塑曼堡塑堕!燮丝茎塑型塑壅堡塑!!翌塑墨塑生堡塑塑型堡旦垫薹ExpressionofCOX一2inovariancancerofthenudemiceandinhibitoryeffectsofCOX——2inhibitorongrowthofovariancancerAbstractObjectiveTostudytheexpressionofcyclooxygenase一2inthetrans—plantedovariancancerofthenudemiceandevaluatetheeffectsoftheselectiveCOX

4、～2inhibitorininducingproliferationOnhumanovariancancerHO-8910cell1ine，andtoobservetheeffectofonthetransplantedovariancancerofthenudemiceinvivo．MethodsTheinhibitoryeffectsofselectiveCOX-2inhibitororlthegrowthofH0-8910werestudiedinvitrobym1method．Meanwhile．theinhibitoryoftumorgrow

5、thintransplantedovariancancerofthenudemicetreatedbyselectireCOX一2inhibitorweredeterminedinrive．TheexpressionofCOX一2andPCNAintransplantedovariancancertissueofthenudemicewasdeterminedbyimmunohisochemisty．TheexpressionofCOX-2intransplantedovariancancertissueofthenudemiceandtheinhib

6、itoryeffectsofselectiveCOX一2inhibitotonthetransplantedovariancancert1ssuewerestudiedbyRl—PCR．Results(1)TheexpressionofCOX一2determinedbyimmunohisochemistywaspositivein3andstronglypositivein7ofthelOtransplantedovariancancertissue．Meanwhile，theexpressionofPCNAdeterminedbyimmunohiso

7、chemistywaspositivein2andstronglypositivein8ofthe10transplantedovariancancertissue．TheexpressionofCOX一2intransplantedovariancancertissuewasstronglydown—regulatedbytheCOX-2在裸鼠卵巢痛移植痛中的袭达及其抑制剂在件内外对卵巢癌嫩长的抑制作用摘要treatmentofSelectiveCOX一2inhibitor(P<0。05)。(2)TheexpressionofCOX-2intrans

8、plantedovariancancertissuedeterminedbyRT—PCRwas