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1、常用大鼠发热模型研究2012年2月第22卷第2期中国比较医学杂志CHINESEJOURNALOFCOMPARATIVEMEDICINEFebrua-,2012Vol.22NO.2常用大鼠发热模型研究左泽平，王志斌，郭玉东,王碧松，高阳(北京市药品检验所，北京100035)【摘要】目的探讨干酵母,2,4■二硝基酚，脂多糖(LPS),细菌内毒索引起SD大鼠实验性发热的过程和特点，比较不同浓度外致热原对大鼠发热过程的影响•方法建立大鼠干酵母(2g/kg,lg/kg),2,4_二硝基酚(30mg/kg,15mg/
2、kg),LPS(100p,g/kg,20g/kg),细菌内毒素(120EU/kg,60EU/kg)发热模型,记录不同时间点大鼠升温值，绘制各模型平均升温曲线，比较不同大鼠发热模型的发热特点.结果皮下注射干酵母混悬液,注射后23h开始升温,6〜7h达峰值，升温持续20h;皮下注射2,4一二硝基酚溶液,注射后20min开始升温,1一1.5h达峰值，升温持续3〜5h;腹腔注射LPS,细菌内毒素，注射后30min开始升温，此后升温曲线表现为双相热或三相热，升温持续5-8h.结论不同外致热原所致SD大鼠发热的过程和
3、特点不同;外致热原浓度不同，所致大鼠发热过程和特点不同.解热试验中，应根据受试药物本身特点选用合适的大鼠发热模型.【关键词】大鼠发热模型;发热过程;发热特点;升温曲线【中图分类号】R33【文献标识码】A【文章编号】1671-7856(2012)02-0052-06doi:10.3969/j.issml671.7856.2012.002.012FeverProcessandCharacteristicsinCommonlyUsedRatFeverModelsZUOZe—ping,WANGZhi—bin,GU
4、OYu—dong,WANGBi—song,GAOYang(BeijinglnstitureforDrugControl,Beijingl00035,China)【Abstract】0bjectiveToexploretheprocessandcharacteristicsoffeverinducedbydryyeast,2,4一dinitrophenolJip0p01ysaccharide(LPS),andbacterialendotoxin(standardsubstance)inSDrats.andt
5、ocomparetheinfluenceofdifferentconcentrationsofexogenouspyrogensonthefeverprocess.MethodsToestablishratfevermodelsinducedbydryyeast(2g/kg,1g/kg),and2,4-dinitrophenol(30mg/kgj5mg/kg),LPS(l00g/kg,20g/kg),andbacterialendotoxin(120EU/kg960EU/kg),recordthefeve
6、rvaluesatdifferenttimepoints.drawtheaveragefevercurves,andcomparetheirfevercharacteristics.ResultsInthefebrileratsinducedbysubcutaneousinjectionofdryyeastsuspension,temperaturebegantoriseafter2-3h,reachedtothepeakvalueafter6-7h?andlastedfor20h.Inthefibril
7、eratsinducedbysubcutaneousinjectionof2,4・dinitrophenolsolution,thetempeniturebegantoriseafter20min,reachedtothepeakvalueafter1一1.5h,andlastedfor3-5h.InthefebrileratsinducedbyintraperitonealinjectionofLPSorbacterialendotoxin(standardsubstance),temperatureb
8、egantoriseafter30min,thenthefevercurveswerebiphasicortriphasic,andlastedfor5hto8h.ConclusionsDifferentexogenouspyrogensatdifferentconcentrationscausedifferentfeverprocessandcharacteristicsinSDrats.lnantipyreticexper
