姜黄素抑制人宫颈癌CaSki细胞裸鼠移植瘤的生长.pdf

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1、2015年2月中国比较医学杂志February，2015第25卷第2期CHINESEJOURNALOFCOMPARATIVEMEDICINEVo1．25No．2姜黄素抑制人宫颈癌CaSki细胞裸鼠移植瘤的生长刘冬菊，姚宇，黄文革(1．广州中医药大学第三附属医院，广州510360；2．中山大学实验动物中心，广州510080)【摘要】目的探讨姜黄素对人官颈癌CaSki细胞裸鼠皮下移植瘤生长的抑制作用。方法建立人宫颈癌裸鼠皮下移植瘤模型，35只荷瘤裸鼠随机分为5组，姜黄素高剂量(200mg／kg)、中剂量(100ms／kg)、低剂

2、量(50ms／kg)组、溶剂对照组和顺铂组(3mg／kg)，每组7只，姜黄素连续灌胃给药15d，顺铂组每3d腹腔注射给药，共5次。停药24h后处死裸鼠，测量裸鼠体重、移植瘤体积、瘤重，计算抑瘤率；光镜下观察移植瘤组织形态学变化；结果处理组的移植瘤体积均明显小于空白对照组(P

3、R33【文献标识码】A【文章编号】1671-7856(2015)02-0007-04doi：10．3969．j．issn．1671．7856．2015．002．002Inhibitoryeffectofcurcuminonthegrowthofhumancervicalcarcinoma(CaSki)xenograftsinnudemiceLIUDong—ju，YAOYu，HUANGWen．ge(1．theThirdAfiliatedHospitalofGuangzhouUniversityofTraditionalChin

4、eseMedicine，Guangzhou510360，China；2．LaboratoryAnimalCenterofZhongshanUniversity，Guangzhou510080)【Abstract】ObjectiveTostudytheinhibitoryeffectofcurcuminonthegrowthofhumancervicalcarcinomaCaSkixenografisinnudemice．MethodsHumancervicalcancercelllineCaSkicellswereinocu

5、latedsubcutaneouslyintonudemice．Thirty—fivetumor—bearingmicewererandomlydividedinto5groups：curcuminhighdose(200mg／kg)，moderatedose(100mg／kg)，lowdose(50ms／kg)，solventcontrolgroupandcisplatingroup(3ms／kg)，7miceineachgroup．Cureuminwasorallyadministeredonceperdayforcon

6、secutive15days．Intraperitonealinjectionofcisplatinwasgiveneverythreedays，foratotaloffivetimes．Themiceweresacrificed24haftertheendoftreatmentandthebodyweight，tumorvolume，tumorweightweremeasuredandtheinhibitionratewascalculated．Thehistologicalchangesoftumortissuewase

7、xamined．ResultsThetumorvolumesofthetreatedgroupweresignificantlylowerthanthatofthecontrolgroup(P<0．01)．Thetumorinhibitionrateofthecurcuminhigh，mediumandlowdosegroupswere44．3％，40．1％and33．3％．respectively．Apparentnecrosiswasobservedinthetumorsofthecurcumin·treatedgrou

8、ps．ConclusionCurcuminpossessesapparentinhibitoryeffectonthegrowthoftransplantedtumorsofhumancervicalcancerCaSkicellsinnudemice．【Keywords】Cervical