两种方法制备水飞蓟宾固体脂质纳米粒的比较

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1、两种方法制备水飞蓟宾固体脂质纳米粒的比较　　作者：厉英超，董蕾，贾皑，苌新明，薛挥【关键词】水飞蓟宾；固体脂质纳米粒；超声学；高压乳匀法　　Comparativestudiesofsilibinincontainedsolidlipidnanoparticlespreparedbytwomethods【Abstract】AIM:Toevaluatethepotentialofthepreparatorymethodsofsolidlipidnanoparticles(SLN)carryingsilibinin(SIL)extractedfromtraditionalChinese

2、medicinalherbs,andtostudythephysicochemicalcharacteristicsoftheSILSLNproducedby2differentmethods.METHODS:SILSLNwaspreparedbyultrasonicationorhighpressurehomogenization.Transmissionelectronmicroscopywasemployedtostudytheshape.Particlecharacterizationsystemandzetapotentialanalyzerwereusedtostud

3、ytheparticlediameterandzetapotentialofSLNinsuspension.Theentrapmentefficiencywasdeterminedbysephadexgelchromatographyandhighperformanceliquidchromatography(HPLC).ThestabilityofSLNwasalsostudied.RESULTS:TheSILSLNpreparedbyultrasonicationwasplateletshapedandirregular,andtheSILSLNpreparedbyhighp

4、ressurehomogenizationwassphericalandregularinshape.TheparticlediametersofSILSLNpreparedbyultrasonicationandhighpressurehomogenizationwere(165±7)nmand(157±6)nmseparately(P<0.05).TheZetapotentialswere(-28.35±2.72)mvand(-35.36±2.68)mvseparately(P<0.001).Theentrapmentefficiencieswere(90.59±

5、0.89)%and(95.64±1.33)%(P＜0.001)separately.SILSLNpreparedbyhighpressurehomogenizationshowedsufficientlongtermstabilitywithonlyslightparticlegrowth(P>0.05)afterstorageatroomtemperaturefor4weeks.CONCLUSION:Highpressurehomogenizationisdemonstratedtobeamoresuitablemethodthanultrasonicationtopre

6、parethesmaller,steadierandhighlyincorporatedSILSLN.【Keywords】silibinin;solidlipidnanoparticles;ultrasonics;highpressurehomogenization【摘要】目的：探索将中药水飞蓟宾（SIL）制备成固体脂质纳米粒（SLN）的方法，并探讨SILSLN的主要性质.方法：分别采用超声法和高压乳匀法制备SILSLN.在电镜下观察其形态，以Mastersizer2000粒度分析仪和ZetasizerNano电位分析仪测定其粒径和Zeta电位，以葡聚糖凝胶柱层析法和HPLC测定

7、其包封率，并观察SLN的稳定性.结果：超声法制备的SILSLN在透射电镜下呈片状存在，形态不规则，高压乳匀法制备的SILSLN呈球状，形态规则.超声法和高压乳匀法制备的SILSLN粒径分别为(165±7)nm和(157±6)nm(P<0.05)；Zeta电位分别为(-28.35±2.72)mv和(-35.36±2.68)mv(P<0.001)；包封率分别为(90.59±0.89)%和(95.64±1.33)%(P<0.001).高压乳匀法制备的SILSLN室温放