外源性p物质对糖尿病大鼠深ii度烧伤创面愈合影响论文

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1、上海交通大学医学院硕士学位论文为对照，在伤后即刻和伤后1、3、7、10、14、21d不同时相点，测量并计算创面愈合率；取不同时相点的创面组织切片作免疫组化染色，结合计算机图像分析，计数创面中性粒细胞、巨噬细胞及新生血管；测定皮肤组织羟脯氨酸（OHP）含量，并作统计学分析。研究结果1.伤后7d起，各时相点A组创面愈合率均明显高于B组。伤后21d，A组创面基本全部再上皮化，而B组创面的愈合率只达到85.44±3.21%。2.A组创面早期炎症细胞（包括PMN和Mφ）浸润量较B组明显增加。在创面愈合后期，B组创面内炎症细胞持续存在。3.伤后7天起，A组创

2、面新生血管化程度较B组均明显增加。4.两组动物于伤后第1d起，OHP含量均下降，之后随着创面的愈合，OHP含量持续上升。A组在伤后7d起各时相点OHP含量均较B组增加。研究结论1.应用外源性P物质可促进糖尿病大鼠深Ⅱ度烧伤创面愈合。2.外源性P物质促进糖尿病烧伤创面愈合的机理可能是：趋化创面中性粒细胞、巨噬细胞的早期浸润；加快新生血管的再生；增加创面胶原蛋白的含量。关键词创面愈合，P物质，糖尿病，烧伤2上海交通大学医学院硕士学位论文THEEFFECTOFEXTERNALSUBSTANCEPONTHEBURNEDWOUNDHEALINGINDIA

3、BETICRATSABSTRACTBackgroundandobjectiveTheintegrityofsensorynervesisanimportantpremiseininflammatoryresponseandtissuerepairandthedisfunctionofsensorynervesoftenleadtothedelayedwoundhealing.Fromthepreviousstudy,weknowthatsubstancePplaysanimportantroleintheprocessofwoundhealing

4、.SubstancePwhichdistributesintheperipheralandcentralnervoussystemwidelyregulatestheinflammatorycells(PMN、Mφ、lymphocyte,etc.)andhasanimpactontheproliferationandtransferenceofrepaircells(keratinocyte,fibroblastandendothelialcell).Furthermore,itplaysanimportantroleinthedevelopme

5、ntoftheperipheralnervesinthewoundhealing.Diabeticneuropathyisacriticalpathologicalbasisfordelayeddiabeticwoundhealing,SubstanceP,oneoftheneuropeptides,secretedfromsensorynerveendingsisanimportantfactorintheprocessofwoundhealing.Inourpreviousstudies,wefoundthatthecontentsofSPi

6、ntheskinofthediabetic3上海交通大学医学院硕士学位论文ratsarelowerdistinctlythanthatinwildrats’skin.Moreover,duringtheprocessofdelayedwoundhealinginthediabeticrats,thelevelofSPinthewoundisdecreasedobviouslycomparedwiththatinthewildrats,whichsuggeststhatthedecreasingSPwouldbeasignificantfactor

7、impactingthediabeticwoundhealing.Inthisstudy,wewouldtrytodemonstratetheeffectoflocalapplicationofSPonthewoundhealinginthediabeticratssufferedfromthepartial-thicknessburnedwound.Methods84WistarratswereinducedintodiabetesbySTZandthenwerereceivedbydeeppartialthicknessburnedwound

8、(thediameteris2cm).Thescaldedratsweredividedintotwogroupsrespectivel