体外团状培养系统中adhtgfβ1诱导骨髓间充质干细胞向软骨细胞的分化

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1、万方数据中国组织工程研究与临床康复多事f3眷多事49勇铲2009—12—03出版JoumelofClinicalRehabilitativeTissueEngineeringResearchDecember3．2009VoL13,No．49跫磊漾基础医学体外团状培养系统中Ad—hTGF一131"司充质干细胞向软骨细胞的分化方军，李华壮，高克海，陈景春ChondrocyticdifferentiationofbonemarrowmesenchymalstemcellsinducedbyAd·hTGF-p1FangJun

2、，LiHua-zhuang，GaoKe—hai，ChenJing-chunAbstractBACKGROUND：Tissueengineenngdevelopmentbringsahopeforarticularcartilagedefectrepair．Currentstudieshavedemonstratedthatbonemarrowmesenchymalstemcells(MSCs)arethebestcellsourcetorepairfu¨-thicknesscartilagedefects．OBJEC

3、TIVE：‘roinducaMSCstodifferentiateintochondrocyteswithAd—hTGF-plinpelletculturesystem／nv／troandidentifythedifferentiatedcells．DESIGN。TIMEANDSEl_rlNG：RepetitivecellularmeasurementswerepedormedattheCentralLaboratoryofXinqiaoHospital。ThirdMilitaryMedicaIUniversityo

4、fChinesePLAfromJune2007toJanuary2008．MATERIALS：Japaneserabbits，2monthsold．wereprovidedbytheLaboratoryAnimalCenterofThirdMilitaryMedicalUniversityofChinesePLA．METHODS：TherabbitMSCswereisolated．culturedandexpanded／nvitro．AftertransfbctedwithAd—hTGF-pl，thecellswer

5、eculturedinpelletculturesystem．Chondrogenicdifferentiationwasevaluatedbyhistological，immunohistochemicelandRT-PCRtechniques．MAIN0UTCOMEMEASURES：Cellmorphologicalchangeswereobsewedbyhistologicalstaining；proteoglycanandtyDe¨collagenexpressionwasdetectedbyimmunohi

6、stochemicaIandRT-PCRtechniques．RESUL-TS：Theinducedcellsexhibitedachondrocyte-likemorphologybyhistologicalstaining．ImmunohistochemicalandRT-PCRresultsshowedthatproteoglycanandtypeIIcollagenwereexpressedintheinducedcalls．CONCLUSl0N：BonemarrowMSCsculturedinpelletc

7、ulturesystemcandifferentiateintochondrocytesundertheinductionofAd·hTGF-81．FangJ，LiHZ，GaoKH，ChenJC．ChondrocyticdifferentiationofbonemarrowmesenchymalstemcellsinducedbyAd-hTGF-B1．ZhongguoZuzhiGongchengYanjiuyuLinchuangKangfu．2009：13(49)：9717-9721．【http：／／www．crte

8、r．cnhttp：llen．zgtckf．cem】DepartmentofOrthopedics．YiduCentraIHospital．Qingzhou262500ShandongProvince，ChinaFangJun，Chiefphysician．DepartmentofOrthopedics．YiduCentraIHospital．Q