临床医学毕业论文h2s对兔离体心脏心率的影响

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1、H2S对兔离体心脏心率的影响姓名：2014年6月25日H2S对兔离体心脏心率的影响作者：陈立锋徐涛范凌赵兰平陈少峰温晓竞张晓云马建伟尹万斌【摘要】0的：探讨硫化氢(H2S)对兔离体心脏心率的影响。方法：用标准微电极细胞内记录技术，观测硫化氢对兔右心房心肌细胞动作电位频率的影响。观测指标：自发放电频率(rateofpacemakerfiring,RPF)。结果：(1)用200(imol/LNaHS灌流时，和正常对照组相比，RPF显著减小(P0.01)。(2)400gmol/LNaHS与对照组比较，

2、RPF显著减小(P0.01)。和200(imol/LNaHS相比，RPF显著减小(P0.01)。⑶以上使RPF减小的效应在NaHS灌流3〜5min出现，lOmin时己经有显著减小(P0.01),25〜30min达到高峰。结论:H2S可浓度依赖性的影响兔离体心脏的心率。【关键词】心率；电生理；硫化氢；心脏；兔Hydrogensu!fide(H2S)hasbeenprovedtobethethirdendogenoussignalinggasotransmitter，besidesnitricoxi

3、de(NO)andcarbonmonoxide(CO).Itshowsimportantphysiologicalfunctionsjnvolvinginthelongtermpotentiationinthehippocampusinthecentralnervoussystem,relaxinggastrointestinaltractsmoothmuscles[l，2].H2Shasbeenalsofoundtoplaymanyregulatingfunctionsincardiovasc

4、ularsystem,andplayacertaineffectinmanycardiovasculardiseases[3].Buttheeffectonheartratehasntbeendeeplyreseached.ByusingstandardintracellularmicroelectrodetechniquetorecordAps，hydrogensulfidewasusedtoinvestigatetherateofpacemakerfiringofleftatrium.The

5、aimofthestudywastoobservetheeffectsofhydrogensulfideonheartrateofrabbitheartsinvitro,elucidatethemechanism(s)involved,provideelectrophysiologicproofforthenosogenesisofsomecardiovasculardiseasesandprovideresearchdirectionsforthedevelopmentofcardiovasc

6、ulardrugs.1MATERIALSANDMETHODS1.1AnimalandReagentsRabbitsofeithersex(2.2±0.2)kg,providedbyExperimentAnimalCenterofHeBeiNorthUniversity.NaHSwaspreparedwithHC1andNa2Saftertheexperimentbegan.1.2SpecimenPreparationRabbitswerekilledwithasingleblowonthehea

7、dandtheheartswerequicklyremovedandplacedinwarm(36.0土0.5)°CimprovedLockesolution(NaCl0.157mol/L,KCl0.56x10-2mol/L,CaCl0.21x10-2mol/L,NaHCO30.18x10-2mol/Landglycose0.56><10-2mol/L).Itwasoxygenatedwith95%O2and5%CO2andmaintainedat(36.0士0.5)°CwithpHof7.40

8、士0.03.Thcrightatriummyocytes(includingsinuatrialnode)werecutfromtherightatriumandthenpinnedonathinsilicondiscatthebottomofaperfusionchamber.Thepreparationswereperfused(5mL/min)withwarm(36.0土0.5)°CimprovedLockesolution.ltwasoxygenatedwith95%O2and5%CO2