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时间:2018-05-01
《h2s对兔离体心脏心率的影响》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、H2S对兔离体心脏心率的影响:陈立锋徐涛范凌赵兰平陈少峰温晓竞张晓云马建伟尹万斌【摘要】目的:探讨硫化氢(H2S)对兔离体心脏心率的影响。方法:用标准微电极细胞内记录技术,观测硫化氢对兔右心房心肌细胞动作电位频率的影响。观测指标:自发放电频率(rateofpacemakerfiring,RPF)。结果:(1)用200μmol/LNaHS灌流时,和正常对照组相比,RPF显著减小(P<0.01)。(2)400μmol/LNaHS与对照组比较,RPF显著减小(P<0.01)。和200μmol
2、/LNaHS相比,RPF显著减小(P<0.01)。(3)以上使RPF减小的效应在NaHS灌流3~5min出现,10min时已经有显著减小(P<0.01),25~30min达到高峰。结论:H2S可浓度依赖性的影响兔离体心脏的心率。【关键词】心率;电生理;硫化氢;心脏;兔Hydrogensulfide(H2S)hasbeenprovedtobethethirdendogenoussignalinggasotransmitter,besidesnitricoxide(NO)andcarbon
3、monoxide(CO).Itshoportantphysiologicalfunctions,involvinginthelongtermpotentiationinthehippocampusinthecentralnervoussystem,relaxinggastrointestinaltractsmoothmuscles[1,2].H2Shasbeenalsofoundtoplaymanyregulatingfunctionsincardiovascularsystem,andplay
4、acertaineffectinmanycardiovasculardiseases[3].Buttheeffectonheartratehasntbeendeeplyreseached.ByusingstandardintracellularmicroelectrodetechniquetorecordAps,hydrogensulfideakerfiringofleftatrium.Theaimofthestudyechanism(s)involved,provideelectrophysi
5、ologicproofforthenosogenesisofsomecardiovasculardiseasesandprovideresearchdirectionsforthedevelopmentofcardiovasculardrugs.1MATERIALSANDMETHODS1.1AnimalandReagentsRabbitsofeithersex(2.2±0.2)kg,providedbyExperimentAnimalCenterofHeBeiNorthUniversity.NaH
6、Sentbegan.1.2SpecimenPreparationRabbitsovedandplacedin(36.0±0.5)℃improvedLockesolution(NaCl0.157mol/L,KCl0.56×10-2mol/L,CaCl0.21×10-2mol/L,NaHCO30.18×10-2mol/Landglycose0.56×10-2mol/L).Itaintainedat(36.0±0.5)℃myocytes(includingsinuatrialnode)therighta
7、triumandthenpinnedonathinsilicondiscatthebottomofaperfusionchamber.ThepreparationsL/min)(36.0±0.5)℃improvedLockesolution.Itaintainedat(36.0±0.5)℃icroelectrodetechniquetorecordAps,thetransmembraneactionpotentialsol/LKClfilledglassmicroelectrode(atipre
8、sistanceof10~20MΩ)coupledtoahighinputimpedenceamplifier(madeinChengduInstrumentfactory),Theamplifiedsignalsultichannelphysiologicalsignalacquisitionsystem(RM6280C,madeinChengduInstrumentFactory)andprocessedbyamicroputer.Theparametersofactionpo
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