重度子痫前期子宫小动脉平滑肌细胞大电导钙激活钾通道的改变

《重度子痫前期子宫小动脉平滑肌细胞大电导钙激活钾通道的改变》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、-Singlechannelcurrentwasamplifiedandfilteredbypatchclampamplifier(CEZ-2200),thenimportedintothecomputer,pClamp6.0softwarewasusedtorecordthedigitaldataandClampfit10.1softwarewasusedtoanalyzedata.ComparethechangeofBKCacurrentsignalintheuterinearterysmoothmusclecellsbetweenthepregnantwomanInn

2、ormalpregnancyandseverepreeclampsia.Results:1.AcuteenzymeseparationofUterinearterialsmoothmusclecells:Thesingleuterinearterialsmoothmusclecellwhichwasdissociatedbyenzymesolutioncanmaintainitsphysiologicalfunctions,anditcanbeusedinpatchclampexperiments2.Thepropertiesofsinglechannelcurrentso

3、fBKCachannelsinUterinearterialsmoothmusclecellswereasfollows:(1)SinglechannelcurrentsofBKCawererecordedininside-outpatch.Thevalueofsinglechannelconductancewas207.2±12.7pS.(2)Thechannelshaddistinctvoltagedependentandcalciumdependentcharacteristics.(3)Inoutside-outpatch(Vm=+30mV),theactivati

4、onofBKCachannelcouldbeblockedby200nMIbTXcompletely.3.comperedthesinglechannelcurrentsofthenormotensivepregnantgroupBKCachannelwiththeseverepreeclampsiagroup:incell-attachedpatch(Vm=+40mV),theThemeanopenprobabilityoftheNormotensivepregnantgroupwas0.7±0.0017,Theamplitudeofvoltagewas7.627±0.7

5、27pA,themeanopentimewas7.216±1.003msms,themeanclosetimewas879.445±149.716ms，theThemeanopenprobabilityoftheSeverepreeclampsiagroupwas0.17±0.0005,Theamplitudeofvoltagewas4.399±0.464pA,themeanopen5----timewas1.642±0.348ms,themeanclosetimewas930.791±264.157ms.comperedwiththeNormotensivepregnan

6、tgroup,thethemeanopentimeandtheamplitudeofvoltageoftheSeverepreeclampsiagroupsignificantlydecreased,thetooftheSeverepreeclampsiagroupsignificantlydecreasedalso.themeanclosetimeoftheSeverepreeclampsiagroupNosignificantdecreased.Conclusion:1.Thisexperimentadoptsthetwo-stepacuteenzymeseparati

7、ontodissociatedpregnancyuterinearterioles,thenwegotthesmoothmusclecellswhichweresuitablefortheperforatedpatchclampexperiment.2.Large-conductanceCa2+-activatedpotassiumchannels(BKCa)arehighlyexpressedinthesmoothmusclecellswhichisolatedfromsmalluterusartery,theB