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1、改良显微缝合线吻合微小血管的实验研究刘麟宋晖廖怡清张勇新周彬黄雪林(广东省深圳市光明新区人民医院外三科518006)【中图分类号】R454【文献标识码】A【文章编号】1672-5085(2012)9-0046-03【摘要】目的探讨改良显微缝合线吻合微小血管的可行性及优点，为微小血管吻合提供一种新的显微缝合线。方法90只雄性SD大鼠，体重250-300g,平均体重为275go随机分为A、B、C三组，每组30只大鼠，A、B两组均建立断尾再植模型进行断尾再植，A组以改良显微缝合线吻合尾动静脉，B组以传统显微缝合线吻合尾动静脉，C组为非手术对照组。记录A
2、、B组血管吻合时间、吻合血管即时通畅率及再植断尾成活例数，术后K14天内每日观察A、B、C组鼠尾的色泽，毛细血管返流时间，皮肤弹性及温度等指标。术后2周行超声Doppler观察A、B组尾动脉通畅情况。结果A组单针吻合时间19±2.5S,单一血管吻合时间11.5±2.5S，吻合血管即时通畅率100%,即时断尾再植成活率100%；B组单针吻合时间29±3.5S,单一血管吻合时间16.4±2.8S,吻合血管即时通畅率100%,断尾再植成活率93.33%。术后2周内，A、B组成活再植鼠尾色泽红润，毛
3、细血管返流时间正常，皮肤弹性正常，与C组无明显差异。A、B组远侧鼠尾皮温稍低于C组，A、B组间皮温无明显差异。超声Doppler显示A、B组成活再植鼠尾之尾动脉血循通畅。结论应用改良显微缝合线吻合微小血管有简易、可靠，缩短血管吻合时间，提高再植断尾成活率等优点。【关键词】微血管吻合显微缝合线改良大鼠断尾再植ExperimentalStudyofAMod讦iedmicro-stitchinMicrovascularAnastomosis,LiuLirSongHui丄iaoYi-Qing(*Departmentofhandsurgery,ThePe
4、ople'sHospitalofGongMing,ShenZhen518006,GuongDon,China)ObjectiveTostudythefeasibilityandadvantagesofamodifiedmicro-stitchinmicrovascularanastomosis,toprovideanovelmicro-stitchinmicrovascularanastomosis.MethodsnintymaleadultSprague-Dawleyratswithanaverageweightof275gweredivide
5、drandomlyintothreegroups,30ratsineverygroup,setupratstailreplantationmodelingroupAandB,microvascularanastomosiswereperformedwithmodifiedmicro-stitchingroupA,microvascularanastomosiswereperformedwithtraditionalmicro-stitchingroupB.Torecordthecompletiontimeofsinglestitchandsing
6、levascularanastomosis,immediatepatencyrateofmicrovascularanastomosis,torecordthenumberofsurvivedreplantedtailsandcalculatedtherateofsurvivalrateofreplantation.TailsofgroupA,B，Cwereinspecteddailyfor14postoperativedaysfortheircolor,capillaryrefiIling,skinconsistency，surfacetemp
7、eratureandwoundcondition.Afterthat,ultrasonicDopplerinspectionweretakentoobservethepatencyofthearteriesingroupA,B.ResultsgroupA:thetimeofsinglestitchwas19±2.5s,thetimeofsinglevascularanastomosiswas11.5±2.5min,theimmediatepatencyrateofmicrovascularanastomosiswas1
8、00%,thesurvivalrateofreplantationwasl00%;groupB:thetimeofsinglestitc
