维药神香草总黄酮对哮喘大鼠气道炎症和气道重塑的影响与其作用机制分析

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1、新疆医科大学硕士学位论文TheeffectandmechanismofTraditionalUyghurMedicinalherbHyssopusofficinalisL．TotalFlavonoidsonairwayinflaromationandremodeHng。ratsmodelnalrwaYinIlammatlonanremoaennginaratsmoelJ●●■J1oICnronlCaStllmaPostgraduate：Ayxam．TaxSupervisor：Prof．Abdiryim．YusupAbstractobjective：Thepro

2、btoHyssopustotalflavonoidsfromcellularandmolecularlevelofasthmaairwayinflammationandairwayremodelingmechanismMethods：(1)Thetotalflavonoidsadsorptionanddesorptioncapacityasindextocompare1differenttypesofmacroporousresinsandScreeningofthebestmacroporousresin，Staticadsorption—elutionte

3、stwerecarriedout．SinglefactortestWasusedtoinvestigatethebestpurificationtechnologyofmacroporousresin．(2)Throughtheadoptionofovalbumin(OVA)injectionatomizationinhalationmethodasthmamodelinrats，observetheeffectofantiasthmaofHyssopustotalflavonoidsonguineapigswerestudied．Bronchialasthm

4、aticmodelWasestablishedbyovialbuminsensitizationandlong—terminhalationprovocation．ThentheratsofeachtreatmentgroupwereintervenewitllthedexamethasoneofHyssopustotalflavonoids．UsingtheFlowtypeintegralplethysmography(whole-bodyplethysmography,WBP)systemdetectedtheeffectsonlungfunction；t

5、heratslungtissuebeingdyedwithHE，Massonweremadepathologicalsection．UsingELISAkitsmethoddetectedthecontentofIgElevel、忻thasthmarats’serumanddetectedthecontentofeotaxinlevel．TGF一131level．CollIIlevelwitllasthmaratslungtissue．Results：(1)AB-8macroporousresinwasthemostsuitableoneforpurifica

6、tionoftotalflavonoidsinHyssopusofficinalisL．Theoptimumpurificationparameterswereasfollowings：thesampleconcentration2．56mg’mL-1，sampleflowrate4BV+h-1，Resinbeddiameterhighratioof1：5，Theresinwaswashedwith5BVwater,theelutionflowrateof5BV／handtheeluentwasdiscardedandelutedwith9BV70％ethan

7、olat4BV．h-1；TheEluateWaSconcentratedanddriedto3新疆医科大学硕士学位论文obtainthetotalflavonoidsformHyssopusofficinalisL．．Thispurificationconditionstomakethetotalflavonoidsconcentrationreached65．6％．(2)Therespiratoryrate(F)inpulmonaryfunctiontestinthemodelgroupincreasedsignificantlycomparedwithno

8、rmalgroup(P<0．oi)，M