低分子硫酸软骨素制备及对软骨修复作用地研究

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1、与模型组相比，给予ChS的组别，其血清中的钙磷含量也相对较少(P

2、通过对上述因子的影响，从而产生积极的作用。硕士研究生裴彤(药物化学)指导教师高华教授关键词：硫酸软骨素；低分子量；降解；软骨修复；炎性因子ResearchoftheMethodforPreparingLowMolecularWeightChondroitinSulfateandEffectofitonCartilageRepairObjectiveAbstractToinvestigatethemethodforpreparinglowmolecularweightchondroitinsulfate，andtoexploretheeff

3、ectofitonCartilageRepair．Methods1、Weighacertainamountofchondroitinsulfate，itsmolecularweightisapproximately50000．DegradingwithHCl，H202andChSaseEACatacertaintemperatureandconditions．Aftercompletionofthereaction，utilizing95％ethanoltoprecipitateanddehydrate．Theproducts’molec

4、ularweightweredeterminedbyhighperformanceliquidchromatography．2、EstablishkneearticularcartilagedefectmodelsonNewZealandrabbits．36NewZealandrabbitswererandomlydividedinto6groups(6ineachgroups)：controlgroup[distilledwater0．49／(kg’d)]，modelgroup[distilledwater0．49／(kg’d)]，lo

5、w—doseoflowmolecularweightChSgroup[10wmolecularweightChS0．29／(kg‘d)]，high—doseoflowmolecularweightChSgroup[10wmolecularweightChS0．49／(kg’d)]，low—doseofhighmolecularweightChSgroup[highmolecularweightChS0．29／(kg’d)]andhigh-doseofhighmolecularweightChSgroup[highmolecularweig

6、htChS0．49／(kg。d)]．Respectivelygavage．Recordingtheweight，survivaltimeandlivingfettleoftherabbitsineachgroup．Observethesituationofcartilagedefectandhistologicalsections．Theexpressionofcytokines，suchasIL一1p，TNF一0【andTGF—pinsynovialfluidwasdetectedbyELISA，determiningcontentof

7、calciumandphosphorusinserumofrabbitbyautomaticbiochemicalanalyzerkit．Resuits1、Determinedbyhighperformanceliquidchromatography,themolecularweightofchondroitinsulfateis55426beforedegrading．Themolecularweightofchondroitinsulfateis3936afterdegradingwithHCl．Thecolorofthesoluti

8、onchangedfromwhitetored-brownintheprocessofdegradation．Theproducts’viscosityislargeanditsyieldis