发酵床不同深度垫料中微生物与温室气体的分布特征-(10298)

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1、ABSTRACT_——————————————————————————————————————————————————————一DISTIRBUTIONOFMICROBEANDGREENHOUSEGASINDIFFERENTDEPTHOFBIO—BEDTherearenumerousmicrobesinthelitterofbio-bed，thesemicrobeswerethemostimportantfactors．ThisstudyusePCR-DGGE，Real-TimePCRtechniqueandi

2、nfraredacousticspectrummonitor，toanalyzeflorasimilarity，thenumberofEscherichiacoli，lactobacillus，methanogens，andconcentrationdistributionofC02，CO，N20，NH3，CH4，H，Oindifferentdepthofbio-bedforthefurtherstudyofcommunityecologyonbio-bed．Duetothecomplexcomposition

3、softhelitterinBio-bed，therearemanyimpuritiesinDNAex臼?act．Underthesecircumstances，theuniversallyapplicableandreproduciblemetllodtoextractDNAfromdifferentorganismsisnotdesirable．Thus，theaimofthisstudywastomodifytheconventionalmethodinordertoextracthighqualityc

4、omplex2enomicDNA行omdifferentbacteriainBio．bed．OurresultsdemonstratedthatmodifiedGenoiseBacteriaDNAEctractionkitCanremovePCRinhibitingfactoreffectively，andtheoptimalconcentrationofttliaminehydrochloridewasO．5M．Throughthetestofbacterialuniversalprimer968f／1401

5、r,bacterial16SrDNAsequences．whichweresamplesfromthelitterinbio—bedofTaicangpigbreedingfarm，wereamplifiedbyPCR,andusingtheDGGEfingerprinttechnologytoanalysetheproductsofthePCR．ItWasfoundthatthenumberofbacterialbandsinahigherlevel(O～15cm)oflitterinbio．bedwassi

6、milarandthefluctuationrangewasfrom24to27．Thenumberofbacterialbandsinthedepthof20cmwasthelowest，whilethenumberinthedepthof30cm陀achedthemaximum，Thesimilaritybetweenthedepthof10cmandotherdepthsoflitterinbio-bedWasthelowest，only51．2％，followedWasthesurfacelayerof

7、padding，andthesimilarityofresiduallevelsWas56．8％；thesimilarityofthesurfaceandthedepthof5cmWastheclosest，reaching95．6％．ByReal．TimePCRtechnology，thispapermadeaquantitativeanalysisofE．coli，LactobacilluSandMethanogensoflitterindifferentdepthsonbio-bed．Thestudyfo

8、undthatthelargestnumberofE．coliwasonthesurfaceofbio-bed，whichWasupto9．68×103cfu．g～．Withthedepthincreasing，thenumberofE．colireduced，menumbrat40cmWasleaSt．帆．、Ⅳecallfoundthatbio．bedcouldeffectively