发酵床不同深度垫料中微生物与温室气体的分布特征

《发酵床不同深度垫料中微生物与温室气体的分布特征》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、ABSTRACT_——————————————————————————————————————————————————————一DISTIRBUTIONOFMICROBEANDGREENHOUSEGASINDIFFERENTDEPTHOFBIO—BEDTherearenumerousmicrobesinthelitterofbio-bed，thesemicrobeswerethemostimportantfactors．ThisstudyusePCR-DGGE，Real-TimePCRtechniqueandinfraredac

2、ousticspectrummonitor，toanalyzeflorasimilarity，thenumberofEscherichiacoli，lactobacillus，methanogens，andconcentrationdistributionofC02，CO，N20，NH3，CH4，H，Oindifferentdepthofbio-bedforthefurtherstudyofcommunityecologyonbio-bed．DuetothecomplexcompositionsofthelitterinBio-

3、bed，therearemanyimpuritiesinDNAex臼?act．Underthesecircumstances，theuniversallyapplicableandreproduciblemetllodtoextractDNAfromdifferentorganismsisnotdesirable．Thus，theaimofthisstudywastomodifytheconventionalmethodinordertoextracthighqualitycomplex2enomicDNA行omdifferen

4、tbacteriainBio．bed．OurresultsdemonstratedthatmodifiedGenoiseBacteriaDNAEctractionkitCanremovePCRinhibitingfactoreffectively，andtheoptimalconcentrationofttliaminehydrochloridewasO．5M．Throughthetestofbacterialuniversalprimer968f／1401r,bacterial16SrDNAsequences．whichwer

5、esamplesfromthelitterinbio—bedofTaicangpigbreedingfarm，wereamplifiedbyPCR,andusingtheDGGEfingerprinttechnologytoanalysetheproductsofthePCR．ItWasfoundthatthenumberofbacterialbandsinahigherlevel(O～15cm)oflitterinbio．bedwassimilarandthefluctuationrangewasfrom24to27．Then

6、umberofbacterialbandsinthedepthof20cmwasthelowest，whilethenumberinthedepthof30cm陀achedthemaximum，Thesimilaritybetweenthedepthof10cmandotherdepthsoflitterinbio-bedWasthelowest，only51．2％，followedWasthesurfacelayerofpadding，andthesimilarityofresiduallevelsWas56．8％；thesi

7、milarityofthesurfaceandthedepthof5cmWastheclosest，reaching95．6％．ByReal．TimePCRtechnology，thispapermadeaquantitativeanalysisofE．coli，LactobacilluSandMethanogensoflitterindifferentdepthsonbio-bed．ThestudyfoundthatthelargestnumberofE．coliwasonthesurfaceofbio-bed，whichWa

8、supto9．68×103cfu．g～．Withthedepthincreasing，thenumberofE．colireduced，menumbrat40cmWasleaSt．帆．、Ⅳecallfoundthatbio．bedcouldeffectively